Later, after the beheading of King Charles in 1649, there was a large influx of cavaliers, who, while they raised the quality of society, much increased the sympathy felt in Virginia for the royal cause. Under their influence Sir William Berkeley denounced the murder of King Charles I., and the General Assembly adopted an act making it treason to defend the late proceedings or to doubt the right of his son, Charles II., to succeed to the crown.[33] Parliament was not long in accepting the challenge which Berkeley tendered. In October, 1650, they adopted an ordinance prohibiting trade with the rebellious colonies of Virginia, Barbadoes, Antigua, and Bermuda Islands, and authorizing the Council of State to take measures to reduce them to terms.[34] In October, 1651, was passed the first of the navigation acts, which limited the colonial trade to England, and banished from Virginia the Dutch vessels, which carried abroad most of the exports. About the same time, having taken measures against Barbadoes, the Council of State ordered a squadron to be prepared against Virginia. It was placed under the command of Captain Robert Dennis; and Thomas Stegge, Richard Bennett, and William Claiborne, members of Berkeley's council, were joined with him in a commission[35] to "use their best endeavors to reduce all the plantations within the Bay of Chesopiack." Bennett and Claiborne were in Virginia at the time, and probably did not know of their appointment till the ships arrived in Virginia. The fleet left England in October, 1651, carrying six hundred men, but on the way Captain Dennis and Captain Stegge were lost in a storm and the command devolved on Captain Edmund Curtis.[36] In December they reached the West Indies, where they assisted Sir George Ayscue in the reduction of Barbadoes. In January, 1652, they reached Virginia, where Curtis showed Claiborne and Bennett his duplicate instructions. Berkeley, full of fight, called out the militia, twelve hundred strong, and engaged the assistance of a few Dutch ships then trading in James River contrary to the recent navigation act.