Tubes of melted agar containing 12 c.c. cooled below 45° C., after receiving 0.6 c.c. of sterile defibrinated horse blood, were inoculated with a loopful of this broth. Thorough mixing and pouring into Petri dishes (10 cm. diameter) followed. After cooling, a second loopful was streaked over the surface of one half of the plate. Deep and superficial planting were thus effected on the same plate. This method was found to be very useful. It can be used with advantage provided one is not called upon to make a great number of cultures when its time consuming factor is a great inconvenience. Another disadvantage is the difficulty of picking single colonies for subculture. In spite of the most careful selection and fishing of a deep colony, subcultures are less likely to be pure than when surface colonies are chosen. By careful regulation of the amount of agar in the tubes, the addition of a measured amount of blood to each enabled one to pour standard blood agar plates. Uniform thorough mixing of the blood is essential so that the plate may present the desired “silky” rather than a “curdled” appearance when viewed by transmitted light. The plates were incubated eighteen to twenty-four hours when subcultures in broth were made from the hemolytic colonies. After growing these for a similar period the additional tests were carried out as indicated above. =Hemolytic Streptococci with Measles.=—The incidence of hemolytic streptococci in the throats of patients with measles admitted to the base hospital at Ft. Riley was found to be remarkably small. TABLE LV HEMOLYTIC STREPTOCOCCI WITH MEASLES IN ALL PATIENTS ADMITTED TO THE WARDS AT CAMP FUNSTON ═══════════════╤════════╤═══════════╤════════╤════════════╤════════════ │ │APPROXIMATE│ NO. OF │ NO. WITH │ PER CENT │DAYS IN │ DAY OF │PATIENTS│ HEMOLYTIC │ WITH │HOSPITAL│ DISEASE │CULTURED│STREPTOCOCCI│ HEMOLYTIC │ │ │ │ │STREPTOCOCCI ───────────────┼────────┼───────────┼────────┼────────────┼──────────── First Culture │ 0 to 1│ 1 to 8│ 112│ 3│ 2.67 Second Culture │ 3 to 10│ 4 to 16│ 86│ 11│ 12.79 Third Culture │ 8 to 23│ 12 to 26│ 58│ 14│ 24.14 ───────────────┴────────┴───────────┴────────┴────────────┴──────────── The first culture represents the findings on admission, in a series of 112 cases; 86 patients being cultured twice; 58 patients three times. Of the 112 cases examined on admission only 3, or 2.67 per cent were found to carry hemolytic streptococci. Those patients who were recultured after from three to ten days in the hospital showed an incidence of 12.8 per cent. A third culture including patients from eight to twenty-three days in the hospital, showed an incidence of 24.1 per cent.