As I had been so fortunate in observing the Lampreys, I felt desirous of ascertaining whether the same thing could be seen in other fish (as in Natural History it is not always safe to reason from analogy), and as there was a large shoal of Minnows spawning near the place where I had seen the Lampreys, I determined to watch their motions. They happened to have chosen a very convenient place for being observed, being between two large stones in the river, which lay about three feet from each other; so that by cautiously approaching them from behind one of the stones, I got close to without disturbing them, but after watching them carefully and repeatedly within the distance of two feet, I can only speak doubtfully of their operations, for they were so numerous, and their motions were so incessant; and when a female was about to shed her spawn, the males (which were ten times more numerous than the females) crowded round her in such a manner as to render it very difficult, if not impossible, to speak with certainty on the subject. I will state what steps I took to satisfy myself, and perhaps the history of my failure may be of use to future observers. It occurred to me from what I observed, that it was probable the males had the power of absorbing the eggs after their exclusion by the female, and impregnating them within their own bodies; and I caught a dozen males at different times, when they were attending on females, and opened them, but I could discover nothing like an egg. I then caught a female, and scattered the spawn (which was expelled by the slightest pressure) in a place frequented by a number of males, but they took no notice of it whatever. I after this caught a female when she was surrounded by a number of males, and apparently in the act of shedding her spawn, and examined whether the spawn which I pressed from her body was impregnated; but it appeared perfectly homogeneous, and so delicate in its texture that it burst with the slightest touch, whilst in that which I picked up from among the gravel where it was scattered abundantly, the impregnation was visible with the assistance of a microscope, and it was so much tougher in its covering as to bear rolling about in my hand without injury. I then tried to impregnate the eggs _mechanically_, and applied a drop of the spermatic fluid to the egg at the moment of exclusion, and it certainly seemed, in one instance, both to increase the size and to alter the colour of the ova it was applied to; but I was not able to produce the same effect so decidedly in any of my subsequent attempts.