In the former part of this experiment, the vessels of the rind have been filled with a solution of lead, that makes of itself no visible alteration in them; but this colourless impregnation, when the orpiment lixivium gets to it, becomes of a deep brown; the vessels themselves appear somewhat the darker for it; but these dots, which are real openings, are now plainly seen to be such, the colour being perfectly visible in them, and much darker than in the vessels. This object must be always viewed dry. If a piece of the rind, thus impregnated, be gently rubbed between the fingers, till the parts are separated, we shall be able in one place or other, to get a view of the vessels all round, and of the films which form the blebs between them. Every part of the rind, and every coat of it, even the interstitial place between its innermost coat and bark, are filled with a fine fluid. The very course and progress of the fluid may be shewn in this part, even by an easy preparation; only that different rinds must be sought for this purpose, the vessels in some being larger than in others. Repeated trials have shewn me that the whole progress may be easily marked in the three following kinds, with only a tincture of cochineal. Put half an ounce of cochineal, in powder, into half a pint of spirit of wine; set it in a warm place, and shake it often for four days; then filter off the clear tincture. Put an inch depth of this into a cup, and set upright in it pieces of the rind of ash, white willow, and ozier, prepared as has been directed, by maceration in water; for in that way one trouble serves for an hundred kinds. Let an inch of the rinds also stand up out of the tincture. After twenty-four hours take them out, clip off the part which was immersed in the fluid, and save the rest for observation. TO PREPARE THE BLEA. Cut the pieces in a fit season, either just before the first leaves of Spring, or in the Midsummer shooting time. Then we see all the wonders of the structure; the thousands of mouths which open throughout the course of these innumerable vessels, to pour their fluid into the interstitial matter. These vessels, which are in nature cisterns of sap for the feeding the growth of the whole tree, are so large, that they are capable of being filled with coloured wax, in the manner of the vessels in anatomical injections; and this way they present pleasing objects for the microscope, and afford excellent opportunities of tracing their course and structure. A METHOD OF FILLING THE SAP VESSELS OF PLANTS.