"The investigations on the effect of the inoculation are made exclusively in those houses in which cholera has actually occurred, the object being to ascertain and compare the incidence of cholera on the inoculated and not inoculated in those houses in which inoculations had been previously carried out. _For this purpose, affected houses in which inoculations have not been performed, and inoculated houses in which cholera has not appeared, are excluded._" Nature gave a demonstration in 77 houses. In one house, and one only, all the household had been inoculated; in 76, inoculated and non-inoculated were living together; but of these 76 houses, 6 are excluded from the table of results, because the inoculated in them were so few--less than one-tenth of the household--that their escape from cholera might be called chance. The cholera came, and left behind it this fact:-- 654 uninoculated individuals had 71 deaths = 10.86 per cent. 402 inoculated in the same households had 12 deaths = 2.99 per cent. If we add the 6 houses which Dr. Simpson excludes, we find that in 77 houses there were 89 deaths from cholera, 77 being among the uninoculated and 12 among the inoculated. Moreover, of these 12 deaths, 5 occurred during the first five days after inoculation--that is to say, during the period in which the protective influence of the vaccine was still incomplete. _Then came a period of more than a year, during which the uninoculated had 42 deaths, and the inoculated had one death._ The remaining 6 of the 12 deaths occurred more than a year after inoculation, and 5 of these 6 had received only one inoculation of the weak vaccine that was used early in 1894. Take a good instance that came at the very beginning of the work:-- "A local epidemic took place around two tanks in Kattal Began _bustee_, ward 19, occupied by about 200 people. In this _bustee_, about the end of March, 2 fatal cases of cholera and 2 cases of choleraic diarrhoea occurred. The outbreak led to the inoculation of 116 persons in the _bustee_ out of the 200. Since then, 9 cases of cholera, of which 7 were fatal, and 1 case of choleraic diarrhoea have appeared in the _bustee_, and it is a very extraordinary fact that all these 10 cases of cholera have occurred exclusively among the uninoculated portion of the inhabitants, which, as stated, forms the minority in the _bustee_; while none of the inoculated have been affected." (_Cholera in Calcutta in 1894._ W. J. Simpson.) 2. _Lucknow_ (1893) The story of the outbreak of cholera in the East Lancashire Regiment must be read carefully:--