2. A person shall not perform on a living animal any experiment calculated to give pain, except subject to the restrictions imposed by this Act. Any person performing or taking part in performing any experiment calculated to give pain, in contravention of this Act, shall be guilty of an offence against this Act, and shall, if it be the first offence, be liable to a penalty not exceeding fifty pounds, and if it be the second or any subsequent offence, be liable, at the discretion of the court by which he is tried, to a penalty not exceeding one hundred pounds, or to imprisonment for a period not exceeding three months. 3. The following restrictions are imposed by this Act with respect to the performance on any living animal of an experiment calculated to give pain; that is to say, (1.) The experiment must be performed with a view to the advancement by new discovery of physiological knowledge or of knowledge which will be useful for saving or prolonging life or alleviating suffering; and (2.) The experiment must be performed by a person holding such license from one of Her Majesty's Principal Secretaries of State, in this Act referred to as the Secretary of State, as is in this Act mentioned, and in the case of a person holding such conditional license as is hereinafter mentioned, or of experiments performed for the purpose of instruction in a registered place; and (3.) The animal must, during the whole of the experiment, be under the influence of some anæsthetic of sufficient power to prevent the animal feeling pain; and (4.) The animal must, if the pain is likely to continue after the effect of the anæsthetic has ceased, or if any serious injury has been inflicted on the animal, be killed before it recovers from the influence of the anæsthetic which has been administered; and (5.) The experiment shall not be performed as an illustration of lectures in medical schools, hospitals, colleges, or elsewhere; and (6.) The experiment shall not be performed for the purpose of attaining manual skill. Provided as follows; that is to say, (1.) Experiments may be performed under the foregoing provisions as to the use of anæsthetics by a person giving illustrations of lectures in medical schools, hospitals, or colleges, or elsewhere, on such certificate being given as in this Act mentioned, that the proposed experiments are absolutely necessary for the due instruction of the persons to whom such lectures are given with a view to their acquiring physiological knowledge, or knowledge which will be useful to them for saving or prolonging life, or alleviating suffering; and (2.) Experiments may be performed without anæsthetics on such certificate being given as in this Act mentioned, that insensibility cannot be produced without necessarily frustrating the object of such experiments; and