_To preserve freshwater polyzoa in an expanded condition._--Place the
polyzoa in a glass tube full of clean water and allow them to expand
their tentacles. Drop on them gradually when they are fully expanded a
2% aqueous solution of cocaine, two or three drops at a time, until
movement ceases in the tentacles. Then pour commercial formaldehyde into
the tube in considerable quantities. Allow the whole to stand for half
an hour. If it is proposed to stain the specimens for anatomical
investigation, they should then be removed through 50% and 70% to 90%
alcohol. If, on the other hand, it is desired to keep them in a
life-like condition they may be kept permanently in a solution of one
part of commercial formaldehyde in four parts of water. Care must be
taken that the process of paralyzing the polypides is not unduly
prolonged, and it is always as well to preserve duplicate specimens in
spirit or formalin with the lophophore retracted.
_To prepare statoblasts for examination._--Place the statoblasts for a
few minutes in strong nitric acid. Then remove the acid with water, pass
through alcohol, clear with oil of cloves, and mount in a small quantity
of Canada balsam under a cover-slip, taking care that the statoblasts
lie parallel to the latter.
ADDENDA.
The following addenda are due mainly to an expedition to the lakes of
Kumaon in the W. Himalayas undertaken by Mr. S. W. Kemp in May, 1911.
PART I.
Genus SPONGILLA.
Subgenus EUSPONGILLA (p. 69).
1 a. Spongilla lacustris, subsp. reticulata (p. 71).
Specimens were taken in the lake Malwa Tal (alt. 3600 feet) in Kumaon,
while others have recently been obtained from the Kalichedu
irrigation-tank in the Pagnor _talug_ of the Nellore district, Madras
(_G. H. Tipper_).
4. Spongilla cinerea (p. 79).
Specimens were taken in Naukuchia Tal (alt. 4200 feet) in Kumaon. They
have a pale yellow colour when dry. This sponge has not hitherto been
found outside the Bombay Presidency.
Subgenus EUNAPIUS (p. 86).
8. Spongilla carteri (p. 87).
Specimens were taken in Bhim Tal (alt. 4450 feet) and Sat Tal (alt. 4500
feet). Some of them approach the variety _cava_ in structure.
Subgenus STRATOSPONGILLA (p. 100).
12. Spongilla bombayensis (p. 102).
Add a new variety:--
13 a. Var. pneumatica, nov.
(i.) The sponge forms a flat layer of a pale brownish colour
as a rule with short and very delicate vertical branches.
In one specimen it takes the form of an elegant cup
attached, only at the base, to a slender twig.
(ii.) The gemmules are covered, outside the spicules, by a
thick pneumatic coat of irregular formation and with
comparatively large air-spaces.
(iii.) The gemmule-spicules are regularly sausage-shaped.
TYPES in the Indian Museum.
HABITAT. Naukuchia Tal (alt. 4200 feet), Kumaon, W. Himalayas (_S. W.
Kemp_).
Genus EPHYDATIA (p. 108).
After _Ephydatia meyeni_, p. 108, add:--
Ephydatia fluviatilis, _auct._
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