But while Galileo was once more partaking of some pleasures, the implacable malice of his enemies never slumbered. There were even some who would have been glad to know that he was for ever safe in the dungeons of the Inquisition. As, however, he gave them no pretext on which they could, with any shadow of justice, have seized him, they had recourse to the most disgraceful means—to lying, anonymous denunciation, in which his enlightened and therefore disliked friend, the Archbishop Ascanio Piccolomini, was ingeniously involved. On 1st February, 1634, the following communication, without signature, was received at the Holy Office at Rome from Siena:— Most Reverend Sirs,— _Galileo has diffused in this city opinions not very Catholic_, urged on by this Archbishop, his host, who has suggested to many persons that Galileo had been unjustly treated with so much severity by the Holy Office, and that he neither could nor would give up his philosophical opinions which he had defended with irrefragable and true mathematical arguments; also that he is the first man in the world, and will live for ever in his works, to which, although prohibited, all modern distinguished men give in their adherence. Now since seeds like these, sown by a prelate of the Church, might bring forth evil fruit, a report is made of them.[484] Although this cowardly denunciation did not bear any immediate consequences either to Piccolomini or Galileo, events which took place soon after show most clearly the unfavourable impression it produced at the Vatican. Galileo, who was very unwell, asked permission of the Pope, through the mediation of his faithful friend Niccolini, to move into Florence for the sake of the regular medical treatment which he required, and which he could not well have at the villa outside the city.[485] As if to dye his tragic fate still darker, just while he was awaiting the result of Niccolini’s efforts, his favourite daughter Polissena, or by her conventual name Marie Celeste, was taken so ill that her life was soon despaired of. It was on one of the last days of March that Galileo was returning to his villa with a physician from a visit to his dying daughter at the Convent of St. Matteo, in deep depression of spirits. On the way the physician had prepared him for the worst by telling him that the patient would scarcely survive till the morning, which proved to be the case. On entering his house in anguish of soul, he found the messenger of the Inquisition there, who in the name of the Holy Office gave him a strict injunction to abstain from all such petitions in future, unless he desired to compel the Inquisition to imprison him again. This unmerciful proceeding had been ordered by a papal mandate of 23rd March.[486] The Inquisitor at Florence reported on it on 1st April to Cardinal Barberini, as follows:—