Attention was directed to the fact that while feeding, fleas are in the habit of squirting blood from the anus and that in the case of those which had fed upon rats and mice dying of the plague, virulent plague bacilli were to be found in such blood. Liston (1905) even found, and subsequent investigations confirmed, that the plague bacilli multiply in the stomach of the insect and that thus the blood ejected was richer in the organisms than was that of the diseased animal. It was found that a film of this infected blood spread out under the body of the flea and that thus the bacilli might be inoculated by the bite of the insect and by scratching. Very recently, Bacot and Martin (1914) have paid especial attention to the question of the mechanism of the transmission of the plague bacilli by fleas. They believe that plague infested fleas regurgitate blood through the mouth, and that under conditions precluding the possibility of infection by dejecta, the disease may be thus transmitted. The evidence does not seem sufficient to establish that this is the chief method of transmission. Conclusive experimental proof that fleas transmit the disease is further available from a number of sources. The most extensive series of experiments is that of the English Plague Commission in India, which reported in 1906 that: On thirty occasions a healthy rat contracted plague in sequence of living in the neighborhood of a plague infected rat under circumstances which prevented the healthy rat coming in contact with either the body or excreta of the diseased animal. In twenty-one experiments out of thirty-eight, healthy rats living in flea-proof cages contracted plague when exposed to rat fleas (_Xenopsylla cheopis_), collected from rats dead or dying of septicæmic plague. Close contact of plague-infected with healthy animals, if fleas are excluded, does not give rise to an epizootic among the latter. As the huts were never cleaned out, close contact included contact with feces and urine of infected animals, and contact with, and eating of food contaminated with feces and urine of infected animals, as well as pus from open plague ulcers. Close contact of young, even when suckled by plague-infected mothers, did not give the disease to the former. If fleas are present, then the epizootic, once started, spreads from animal to animal, the rate of progress being in direct proportion to the number of fleas. Aerial infection was excluded. Thus guinea-pigs suspended in a cage two feet above the ground did not contract the disease, while in the same hut those animals allowed to run about and those placed two inches above the floor became infected. It had previously been found that a rat flea could not hop farther than about five inches.