than the royal nymph was dislodged from its cell, and by the Bees brought out of the pavilion, and laid lifeless on the front-board. This fact taught me by experiment, that the reigning Queen would very soon, from real necessity, have been compelled to leave the now discarded nymph to take possession of the hive. The Queen, owing to the excessive and daily increasing heat of the hive, would have left her wealthy colony--would have been compelled to leave it--had not the ventilation, and the enlargement of her domicil, prevented the painful necessity of her so doing. This, I think, proves the truth of the observation--that it is the old Queen which leaves, when Bees are compelled to swarm; but, if not, the following experimental operations have demonstrated the fact. I have united many swarms, and every sovereign Bee I have been under the necessity of making a captive, has invariably been an old one. On the 25th of June, 1828, I took up a parent-stock, four days after it had thrown off a swarm, and there found only the royal nymph within its cradle--_there was no Queen left in that stock, save the one in embryo_--the old Queen had gone with the swarm. This lesson caused me to carry my experiments farther. Having taken up the parent-stock, as just stated, I united all the working Bees of that stock to those of the swarm already mentioned, and I also put the young larvæ found in the parent-stock, to the now united-stock; I then placed the intended royal species--the nymph already mentioned--with the remainder of the young brood, in one of the collateral-boxes, and immediately let the odour of the stock through the communicating slide. To my great satisfaction I discovered the willingness of the old Bees to bring to perfection the young they had been compelled to leave in their former domicil. The royal nymph, however, was an exception; she alone was instantly dragged from her cell, and cast out of the hive.