Impurities are much commoner and more abundant in the air of enclosed spaces than in the external air, as the natural processes of purification cannot be brought to bear so efficiently in the former case. In sick rooms, hospitals, etc., impurities arise, which are not present where only healthy people are collected. The most important impurities are derived from the respiration of animals, and the combustion of gases, candles, or lamps in rooms, from sewage emanations, from various occupations, and the air of marshes, mines, church-yards, etc. These may be classed under two heads—_solid_ and _gaseous_; the solid being simply suspended in the air in a finely divided condition, or floated about in a coarser condition by currents of air. They are revealed in an atmosphere in which one did not previously suspect their existence, by the passage of a beam of sunlight. Light itself is invisible, but its course is rendered visible by the particles from which its rays are reflected. Tyndall demonstrated the presence of minute particulate matter in the air of all ordinary situations, and showed that a large proportion of this matter consists of germs (microbes). In his experiments with vapours in closed tubes, floating matter was always revealed by a concentrated beam of light, even though the air entering the tube had been first drawn through sulphuric acid and through a strong solution of caustic potash. If this air was then passed through a red-hot platinum tube and across folds of red-hot platinum gauze, it became _optically empty_; the floating matter had been burnt, and disappeared. It was therefore organic. In subsequent experiments, he took organic solutions, as of meat, turnip, and the like, and rendered them sterile by repeated boiling. They remained sterile when kept in air-tight vessels or in vessels covered with a thick layer of cotton-wool, which would efficiently filter any entering air; but when exposed to the air, they invariably became turbid, owing to an enormous multiplication of germs. Clearly, therefore, air contains organic, matter, and much of this organic matter consists of living germs. Most of these germs are comparatively harmless under ordinary conditions. They are, however, the causes of fermentation, putrefaction, and all the processes of decomposition which occur in organic substances. The importance of the exclusion of the dust of air has received an important application in _Lister’s antiseptic_ and in the _aseptic_ system of treatment of wounds. Formerly accidents and operations were frequently fatal; now vast numbers of lives are saved by improved surgical methods. The original _antiseptic_ method acted on the supposition that some germicidal application to the wounds was necessary; now it is realized that if, during the operation, germs are not allowed to remain in the wound, all that is afterwards necessary to insure rapid recovery is that they shall be prevented from entering the wound from the external