Second son of Robert Rookwood of Stanningfield, by his second wife Dorothy, daughter of Sir William Drury of Hawkstead; he became eventually the heir of his father. Ambrose was born in 1578, and was educated in Flanders as a Roman Catholic. According to Greenway, he was "beloved by all who knew him;" Gerard describes him as "very devout, of great virtue and valour, and very secret; he was also of very good parts as for wit and learning." He was remarkable for his stud of fine horses. Coldham Hall, his family mansion, built by his father in 1574, is still standing, and is a picturesque house, about four miles from Bury Saint Edmunds. Very reluctant at first to join the plot, (March 31st, 1605), when arrested he "denied all privity, on his soul and conscience, and as he was a Catholic." He was drawn into it by Catesby, with whom he had long been acquainted, and whom he said that he "loved and respected as his own life." Objecting that "it was a matter of conscience to take away so much blood," Catesby replied that he was "resolved that in conscience it might be done," whereon Rookwood, "being satisfied that in conscience he might do it, confessed it neither to any ghostly father nor to any other." (Exam, of Rookwood, _Gunpowder Plot Book_, article 136.) Sir William Wade writes that "Rookwood can procure no succour from any of his friends in regard of the odiousness of his actions," (Additional Manuscript 6178, folio 34). He seems to have been fond of fine clothes, for he not only had a "fair scarf" embroidered with "ciphres," but "made a very fair Hungarian horseman's cote, lyned all with velvet, and other apparel exceeding costly, not fyt for his degree," (_Ibidem_, folio 86). His wife, who was "very beautiful" and "a virtuous Catholic," was the daughter of Robert Tyrwhitt, Esquire, of Kettleby, county Lincoln. They had three children: Sir Robert Rookwood, who warmly espoused the cause of Charles the First, and was buried 10th June, 1679; he married Mary, daughter of Sir Robert Townsend of Ludlow, and left issue: Henry: and Elizabeth, wife of William Calverley, Esquire. The Rookwoods of the Golden Fish, in the story, are all fictitious persons. The real brother of Ambrose was the Reverend Thomas Rookwood of Claxton, the correspondent of Garnet. FRANCIS TRESHAM.