1. _Chloroform._--My observations with regard to the distribution of nerves in Sarsia led me to investigate the order in which these connections are destroyed, or temporarily impaired, by anæsthetics. The results, I think, are worth recording. In Sarsia the following phases always mark the progress of anæsthesia by chloroform, etc.--1. Spontaneity ceases. 2. On now nipping a tentacle, pulling the manubrium, or irritating the bell, a _single_ locomotor contraction is given in answer to every stimulation. (In the unanæsthesiated animal a _series_ of such contractions would be the result of such stimulation.) 3. After locomotor contractions can no longer be elicited by stimuli, nipping a tentacle or the margin of the bell has the effect of causing the manubrium to contract. 4. After stimulation of any part of the nectocalyx (including tentacles) fails to produce response in any part of the organism, the manubrium will continue its response to stimuli applied directly to itself. 2. _Nitrite of Amyl._--On Sarsia the effect of this agent is much the same as that of chloroform--the description just given being quite as applicable to the affects of the nitrite as to those of chloroform. Before the loss of spontaneity supervenes, the rate of the rhythm is increased, while the strength of the pulsations is diminished. Tiaropsis diademata, from the fact of its presenting a very regular rhythm and being but of small size, is a particularly suitable species upon which to conduct many experiments relating to the effect of poisons. On this species the nitrite in appropriate (_i.e._ in very small) doses first causes irregularity and enfeeblement of the contractions, together with quickening of the rhythm. After a short time, a gradual cessation of the swimming motions becomes apparent--these motions dying out more gradually, for example, than they do under the influence of chloroform. Eventually each pulsation is marked only by a slight contraction of the muscular tissue in the immediate neighbourhood of the margin. If the dose has been stronger, however, well-marked spasmodic contractions come on and obliterate such gradual working of the poison. In all cases irritability of all parts of the animal persists for a long time after entire cessation of spontaneous movements--perhaps for three or four minutes in not over-poisoned animals; but eventually it too disappears. On being now transferred to normal sea-water, the process of recovery is slower than it is after anæsthesiation by chloroform. It is interesting, moreover, to observe, that just as the power of co-ordination was the first thing to be affected by the nitrite, so it is the last thing to return during recovery.