If bees are to be made to enter a new hive, by removing the old one from its stand, it will always be very desirable not only to have the new one contain a piece of comb, but a considerable number of bees _clustered_ on that comb. I repeatedly found my bees, after entering the hive, refuse to have anything to do with the brood comb, and for a long time, I was unable to conjecture the cause; until I ascertained that they were dissatisfied with its deserted appearance, and that, by taking the precaution to have it well covered with bees, I seldom failed to reconcile them to my system of forced colonization. I can usually tell, in less than two minutes, whether the operation will succeed or not. If the returning bees are content, they will, however much agitated at first, soon begin to join the cluster on the comb; while if they are dissatisfied, they will abandon the hive, and nearly all the bees that were originally on the comb, will leave with them. They seem capricious in this matter, and are sometimes so very self-willed, that they refuse to have anything to do with the brood comb, when I can see no good reason why they should be so rebellious. I shall here state some _conjectures_ which have occurred to me on this subject. Is it absolutely certain that bees can raise a queen from _any_ egg or young larva which would produce a worker? Or if this is possible, is it certain that _any kind of workers_ can accomplish this? Huber ascertained to his own satisfaction that there were two kinds of workers in a hive. He thus describes them. "One of these is, in general, destined for the elaboration of wax, and its size is considerably enlarged when full of honey; the other immediately imparts what it has collected to its companions, its abdomen undergoes no sensible change, or it retains only the honey necessary for its own subsistence. The particular function of the bees of this kind is to take care of the young, for they are not charged with provisioning the hive. In opposition to the wax workers, we shall call them small bees or nurses." "Although the external difference be inconsiderable, this is not an imaginary distinction. Anatomical observations prove that the capacity of the stomach is not the same--experiments have ascertained that one of the species cannot fulfil all the functions shared among the workers of a hive. We painted those of each class with different colors, in order to study their proceedings; and these were not interchanged. In another experiment, after supplying a hive deprived of a queen with brood and pollen, we saw the small bees quickly occupied in nutrition of the larvæ, while those of the wax working class neglected them. Small bees also produce wax, but in a very inferior quantity to what is elaborated by the real wax workers."