each one, and it should contain no old bees, but brood rapidly maturing, and if possible, eggs and worms only a few days old. This new addition of strength will greatly encourage the nuclei, and give them the means of starting young queens, if they have not succeeded in doing so with the first comb. I have very frequently found that for some cause which I have not yet ascertained, they often start a large number of queen cells, which in a few days, are all discontinued and untenanted. The second attempt seldom fails. Does practice in this thing make them more expert? But I will simply state the fact, referring to my conjectures on page 218; and remarking that when they make a second attempt, they seem frequently disposed to start a much larger number than they otherwise would have done. In two or three days after giving them the first piece of comb, I give them another, if their queen is nearly mature, and I now let them alone until she ought to be depositing eggs in the hive. I then give them, at intervals of a few days, two or three combs more, and they will now be sufficiently powerful in bees, to gather large quantities of honey, and fill the empty part of their hive. The young queen is supplying with thousands of worker-eggs, the cells from which the brood has emerged, and also the new ones built by the bees, and the new colony will soon be one of the best stock hives in the Apiary. If some of the full frames are moved, and empty ones placed between them, as soon as the bees begin to build powerfully, there need be no guide combs on the empty frames, and still the work will be executed with the most beautiful regularity.