About a week after the young queens have hatched, I examine all the hives which contain them, lifting out usually, some of the largest combs, and those which ought to contain brood. If I find a comb which has eggs or larvæ, I am satisfied that they have a fertile queen, and shut up the hive; unless I wish to find her, in order to deprive her of her wings, (see p. 203.) I can thus often satisfy myself in one or two minutes. If no brood is found, I suspect that the queen has been lost, or that she has some defect which has prevented her from leaving the hive. If the brood-comb which I put into the hive, contains any newly-formed royal cells, I _know_, without any further examination, that the queen has been lost. If the weather has been unfavorable, or the colony is quite weak, the young queen is sometimes not impregnated as early as usual, and an allowance of a few days must be made on this account. If the weather is favorable, and the colony a good one, the queen usually leaves, the day after she finds herself mistress of a family. In about two days more, she begins to lay her eggs. By waiting about a week before the examination is made, ample allowance, in most cases, is made. Early in the month of September, I examine carefully all my hives, so as to see that in every respect, they are in suitable condition for wintering. If any need feeding, (See Chapter on Feeding,) they are fed at this time. If any have more vacant room than they ought to have, I partition off that part of the hive which they do not need. I always expect to find some brood in every healthy hive at this time, and if in any hive I find none, and ascertain that it is queenless, I either at once break it up, or if it is strong in numbers supply it with a queen, by adding to it some feebler stock. If bees, however, are properly attended to, at the season when their young queens are impregnated, it will be a very rare occurrence to find a queenless colony in the Fall.