In 1635 he was deprived of his charge as minister of the united parishes of Kirkdale and Kirkmabreck. In 1637, when Episcopacy began to be the losing cause, he returned to his flock. His name appears on the roll of the members of the famous Assembly which met at Glasgow in 1638; and in 1639 he was translated to Cramond, as successor to Mr. William Colville, afterwards Principal of the University of Edinburgh; to whom he appears to have been related, as the name of his wife was Elizabeth Colville. He was the intimate friend of the well-known Alexander Henderson, who by his latter will ordained his executor "to deliver to my dear acquaintance Mr. John Duncan, at Culross, and Mr. William Dalgleish, minister at Cramond, all my manuscripts and papers which are in my study, and that belong to me any where else; and after they have received them, to destroy or preserve and keep them, as they shall judge convenient for their own private or the public good." In 1662 Dalgleish was ejected for nonconformity, and died before the Revolution. _Kirkmabreck_ was a pendicle of the Abbey of Dundrennan, which is seven miles from Kirkcudbright. The farms and cottages that bear this name are about two miles from the shore, a little way up on the high ground, but the church and churchyard lie in a hollow, between the Larg and the Cairnharrow hills. Part of the old ivy-covered walls, and the gable of the church, still remain. One modern tomb in the churchyard is marked by a granite pillar, 20 feet high. It is the grave of Dr. Thomas Brown. The inscription on the west side reads thus:--"Thomas, M.D., Professor of Moral Philosophy in the University of Edinburgh, who died 2nd August 1820, aged 43 years. Janet, who died 5th August 1824, aged 51." The Statistical Account speaks of Old Mortality having renovated some of the grave-stones, but all traces of his work have disappeared. In that old church Samuel Rutherford preached his sermons on Zech. xiii. 7, 9, at a Communion in 1630. In 1634 he preached on Luke xiv. 16, at the preparation before the Communion; and on another occasion, on Isaiah xlix. 1-4.