When I published the sixth edition of the 'Origin,' I thought a good deal on the subject to which you refer, and the opinion therein expressed was my deliberate conviction. I went as far as I could, perhaps too far in agreement with Wagner; since that time I have seen no reason to change my mind, but then I must add that my attention has been absorbed on other subjects. There are two different classes of cases, as it appears to me, viz. those in which a species becomes slowly modified in the same country (of which I cannot doubt there are innumerable instances) and those cases in which a species splits into two or three or more new species, and in the latter case, I should think nearly perfect separation would greatly aid in their "specification," to coin a new word. I am very glad that you are taking up this subject, for you will be sure to throw much light on it. I remember well, long ago, oscillating much; when I thought of the Fauna and Flora of the Galapagos Islands I was all for isolation, when I thought of S. America I doubted much. Pray believe me, Yours very sincerely, CH. DARWIN. P.S.--I hope that this letter will not be quite illegible, but I have no amanuensis at present. CHARLES DARWIN TO K. SEMPER. Down, November 30, 1878. Dear Professor Semper, Since writing I have recalled some of the thoughts and conclusions which have passed through my mind of late years. In North America, in going from north to south or from east to west, it is clear that the changed conditions of life have modified the organisms in the different regions, so that they now form distinct races or even species. It is further clear that in isolated districts, however small, the inhabitants almost always get slightly modified, and how far this is due to the nature of the slightly different conditions to which they are exposed, and how far to mere interbreeding, in the manner explained by Weismann, I can form no opinion. The same difficulty occurred to me (as shown in my 'Variation of Animals and Plants under Domestication') with respect to the aboriginal breeds of cattle, sheep, etc., in the separated districts of Great Britain, and indeed throughout Europe. As our knowledge advances, very slight differences, considered by systematists as of no importance in structure, are continually found to be functionally important; and I have been especially struck with this fact in the case of plants to which my observations have of late years been confined. Therefore it seems to me rather rash to consider the slight differences between representative species, for instance those inhabiting the different islands of the same archipelago, as of no functional importance, and as not in any way due to natural selection. With respect to all adapted structures, and these are innumerable, I cannot see how M. Wagner's view throws any light, nor indeed do I see at all more clearly than I did before, from the numerous cases which he has brought