This is the date of the first observation on the presence of little parasitic bodies in splenic fever, but, strange to say, no attention was paid to these minute filaments. Rayer and Davaine also paid no attention to them. This indifference lasted for thirteen years; it would have lasted longer still, if the parasitic origin of communicable diseases had not been brought before the mind by each new publication of Pasteur's. From 1857 to 1860 it will be remembered that he had demonstrated lactic fermentation, like alcoholic fermentation, to be the work of a living ferment; in 1861 he had discovered that the agent of butyric fermentation consisted of little moving thread-like bodies, of dimensions similar to those of the filaments discovered by Davaine and Rayer in the blood of splenic fever patients; in 1861 he had announced that no ammoniacal urine existed without the presence of a microscopic organism; in 1863 he had established that the bodies of animals in full health are sealed against the introduction of the germs of microscopic organisms; that blood drawn with sufficient precaution from the veins and the arteries, and urine taken direct from the bladder, could be exposed to the contact of pure air without putrefaction, and without the appearance of living thread-like organisms of any kind whatever, mobile or immobile. It was all these facts which in 1863 brought back the attention of Davaine, as he himself has acknowledged, to the observation which he had made in 1850. 'M. Pasteur,' said M. Davaine in a communication made to the Academy of Sciences, 'published some time ago a remarkable memoir on butyric fermentation, which consists of little cylindrical rods, possessing all the characteristics of vibrios or of bacteria. The thread-like corpuscles which in 1850 I saw in the blood of sheep attacked with _sang-de-rate_, having a great analogy with these vibrios, I was led to examine whether filiform corpuscles, analogous to or of the same kind as those which determined the butyric fermentation, would not, if introduced into the blood of an animal, equally act the part of a ferment. Thus would be easily explained the alteration, and the rapid infection of the mass of the blood, in an animal which had received accidentally or experimentally into its veins a certain number of these bacteria--that is to say, of this ferment.' But two summers passed before M. Davaine was able to procure a sheep affected with the _sang-de-rate_. It was only in 1863 that he first recognised the constant presence of a parasite, in the blood of sheep and rabbits which had died from successive inoculations with blood taken after death or in the last hours of life. He further proved that the inoculated animal, in the blood of which no parasites were as yet visible with the microscope, had every appearance of health, and that in these conditions the blood could not communicate splenic fever.