It was on February 28, 1881, that Pasteur communicated to the Academy of Sciences, in his own name, and in those of his two fellow workers, the exposition of this great discovery. Loud applause burst forth with patriotic joy and pride. And yet so marvellous were these results that some colleagues could not help saying, 'There is a little romance in all this.' All this reminds one, in fact, of what the alchemist of Lesage did to the demons which annoyed him. He shut them up in little bottles, well corked, and so kept them imprisoned and inoffensive. Pasteur shut up in glass bulbs a whole world of microbes, with all sorts of varieties which he cultivated at will. Virulences attenuated or terrible, diseases benign or deadly, he could offer all. Hardly had the journals published the _compte-rendu_ of this communication when the President of the Society of Agriculture in Melun, M. le Baron de la Rochette, came, in the name of the Society, to invite Pasteur to make a public experiment of splenic fever vaccination. Pasteur accepted. On April 28 a sort of convention was entered into between him and the Society. The Society agreed to place at the disposal of Pasteur and his two young assistants, Chamberland and Roux, sixty sheep. Ten of these sheep were not to receive any treatment; twenty-five were to be subjected to two vaccinal inoculations at intervals of from twelve to fifteen days, by two vaccines of unequal strength. Some days later these twenty-five sheep, as well as the twenty-five remaining ones, were to be inoculated with the virus of virulent splenic fever. A similar experiment was to be made upon ten cows. Six were to be vaccinated, four not vaccinated; and the ten cows were afterwards, on the same day as the fifty sheep, to receive inoculation from a very virulent virus. Pasteur affirmed that the twenty-five sheep which had not been vaccinated would perish, while the twenty-five vaccinated ones would resist the very virulent virus; that the six vaccinated cows would not take the disease, while the four which had not been vaccinated, even if they did not die, would at least be extremely ill. As soon as the agricultural and scientific press had published this programme, and recorded Pasteur's prophecies, several of his colleagues at the Academy of Sciences, startled by such boldness in reference to a subject which had hitherto been enveloped in such profound obscurity, and fearing to see the illustrious company somewhat compromised by these affirmations in relation to problems of physiology and pathology, addressed some observations to M. Pasteur on what they called 'a scientific imprudence.' 'Take care,' they said to him, 'you are committing yourself without possibility of retreat. Your experiments in the laboratory hardly authorise you to attempt experiments like those at Melun.'