At the time here referred to, the Minister of Public Instruction said to Pasteur, 'There is no clause in the budget to grant you 1,500 francs a year to defray the expense of experiments.' Pasteur did not hesitate to establish a laboratory at his own expense in one of the garrets of the École Normale. We can readily imagine the modesty of such an establishment in such a place. Dividing his time between his professional duties and his laboratory experiments, Pasteur never went out but to talk over his daily researches with M. Biot, M. Dumas, M. de Senarmont, and M. Balard. M. Biot especially was his habitual confidant. The day when M. Biot learned that Pasteur proposed to study the obscure question of spontaneous generation, he strongly dissuaded him from entangling himself in this labyrinth. 'You will never escape from it,' said he, 'you will only lose your time;' and when Pasteur attempted some timid observations with the view of showing that in the order of his studies it was indispensable for him to attack this problem, M. Biot grew angry. Although endowed, as Sainte-Beuve has said, with all the qualities of curiosity, of subtlety, of penetration, of ingenious exactitude, of method, and of perspicuity, with all the qualities, in short, essential and secondary, M. Biot treated the project of Pasteur as a presumptuous adventure. Bolder than M. Biot, but with a circumspection always alive, M. Dumas declared to Pasteur, without, however, further insisting upon the point, that he would not advise anyone to occupy himself too long with such a subject. M. de Senarmont alone took the part of Pasteur, and said to M. Biot: 'Let Pasteur alone. If there is nothing to be found in the path which he has entered upon, do not be alarmed, he will not continue in it. But,' added he, 'I should be surprised if he found nothing in it.' M. Pouchet had previously stated the problem with precision: 'The opponents of spontaneous generation assert that the germs of microscopic organisms exist in the air, which transports them to a distance. What, then, will these opponents say if I succeed in inducing the generation of living organisms, while substituting artificial air for that of the atmosphere?'