Louis Pasteur: His Life and LaboursVallery-Radot, René
Science
Louis Pasteur: His Life and Labours
Vallery-Radot, René
Pasteur, Louis, 1822-1895; Scientists -- France -- Biography
'If we had time to examine them well,' continued Pasteur, 'we should
see them, though agitated with various movements, falling downwards
more or less quickly. It is thus that all objects become covered with
dust--the furniture, the table, the mercury in this basin. Since this
mercury was taken from the mine, how much dust must have fallen upon
it, to say nothing of all that has been intimately mixed up with it
during the numerous manipulations to which it has been subjected in the
laboratory? It is not possible to touch this mercury, to place the hand
in it, or a bottle, without introducing into the interior of the basin
the dust which lies on its surface. You will now see what takes place.'
Projecting, in the darkness, the beam of light upon the basin of
mercury, the liquid metal shone forth with its usual brilliancy.
Pasteur then sprinkled some dust upon the mercury, and, plunging a
glass rod into it, the dust was seen to travel towards the spot where
the rod entered the mercury, and to penetrate into the space between
the glass and the metal.
'Yes,' exclaimed Pasteur with a voice which gave evidence of the
sincerity of his conviction, 'yes, M. Pouchet had removed the germs
from the water and from the hay, but he had neglected to remove the
dust from the surface of the mercury. This is the cause of his error;
this is what has vitiated his whole arrangement.'
Pasteur then instituted experiments exactly similar to those of
Pouchet, but taking care to remove every cause of error which had
escaped the latter. He employed a glass bulb with a long neck, which
he bent, and connected with a tube of platinum placed in a furnace, so
that it could be heated nearly to redness. In the bulb he placed some
very putrescible liquids--urine for example. When the furnace which
surrounded the platinum tube was in action, Pasteur boiled the liquid
for some minutes, then he allowed it to cool, keeping the fire around
the platinum tube still active. During the cooling of the bulb the
external air was introduced, after having first travelled through the
red-hot platinum tube. The liquid was thus placed in contact with air
whose suspended germs were all burnt up.
In an experiment thus carried out, the urine remains unchanged--it
undergoes only a very slight oxidation, which darkens its colour a
little--but it exhibits no kind of putrefaction. If it be desired
to repeat this experiment with alkaline liquids, such as milk, the
temperature must be raised a little above the boiling point--a
condition easily realised with the apparatus just described. It is
only necessary to connect with the free extremity of the platinum
tube a glass tube bent at right angles, and to plunge the latter
to a depth of some centimeters into a basin of mercury. In these
circumstances ebullition goes on under a pressure greater than that of
the atmosphere, consequently at a temperature higher than 100 degrees
Centigrade.
Public-domain text, read in full here on John Shaqi.
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