The product of the bees, second in importance, is wax. This is a solid, unctious substance, and is, as shown by its chemical composition, a fat-like material, though not as some authors assert, the fat of bees. As already observed, this is a secretion formed in pellets, the shape of an irregular pentagon (Fig. 27, _w_), underneath the abdomen. These pellets are light-colored, very thin and fragile, and are secreted by and molded upon the membrane towards the body from the wax-pockets. Neighbour speaks of the wax oozing through pores from the stomach. This is not the case, but, as with the synovial fluid about our own joints, is formed by the secreting membrane, and does not pass through holes, as water through a sieve. There are four of these wax-pockets on each side, and thus there may be eight wax-scales on a bee at one time. This wax can be secreted by the bees, when fed on pure sugar, as shown by Huber, which experiment I have verified. I removed all honey and comb from my observing-hive, left the bees for twenty-four hours to digest all food which might be in their stomachs, then fed pure sugar, which was better than honey, as Prof. R. F. Kedzie has shown by analysis that not only filtered honey, but even the nectar which he collected right from the flowers themselves, contains nitrogen. The bees commenced at once to build comb, and continued for several days, so long as I kept them confined. This is, as we should suppose; sugar contains hydrogen and oxygen in proportion to form water, while the third element, carbon, is in the same or about the same proportion as the oxygen. Now, the fats usually contain little oxygen, and a good deal of carbon and hydrogen. Thus, the sugar by losing some of its oxygen would contain the requisite elements for fat. It was found true in the days of slavery in the South, that the negroes of Louisiana, during the gathering of the cane, would become very fat. They ate much sugar; they gained much fat. Now, wax is a fat-like substance, not that it is the animal fat of bees, as often asserted--in fact it contains much less hydrogen, as will be seen by the following formula from Hess: Oxygen 7.50 Carbon 79.30 Hydrogen 13.20 --but it is a special secretion for a special purpose, and from its composition, we should conclude that it might be secreted from a purely saccharine diet, and experiment confirms the conclusion. It has been found that bees require about twenty pounds of honey to secrete one of wax. That nitrogenous food is necessary, as claimed by Langstroth and Neighbour, is not true. Yet, in the active season, when muscular exertion is great, nitrogenous food must be imperatively necessary to supply the waste, and give tone to the body. Some may be desirable even in the quiet of winter. Now, as secretion of wax demands a healthy condition of the bee, it indirectly requires some nitrogenous food.