The last of these interesting laboratory experiments on the rate of nitrification we shall refer to, are those by Deherain. He experimented with soils containing different amounts of nitrogen and moisture. With a soil containing .16 per cent of nitrogen he obtained, during a period of 90 days, rates of nitrification varying from .71 to 1.09 per million parts of soil. The maximum quantity was formed when the soil contained 25 per cent of moisture. On a soil considerably richer--viz.,.261 per cent of nitrogen--a higher rate of nitrification took place--1.48 parts per million. The highest rate obtained in these experiments showed, when calculated to pounds per acre, about 5-1/2, taking the soil to a depth of 9 inches. When the soil was alternately dried and moistened the process was most rapid. _Portion of Soil-nitrogen more easily Nitrifiable than the rest._ Lastly, it may be noticed that in the above-cited experiments, and others of a similar kind, the process goes on most rapidly at first, and steadily diminishes thereafter. This is due to the fact, that there is generally a certain quantity of nitrogen in most soils in a more easily nitrifiable condition than the rest, so that when this becomes oxidised nitrification proceeds more slowly. It would further seem that the nitrogen of the subsoil is less easily nitrified than that of the surface-soil. _Rate of Nitrification deduced from Field Experiments._ While the above experiments throw much light on the question of the rate at which nitrification may go on under different circumstances, the results furnished by actual analyses of soils and their drainage-waters are of still more practical value; and the Rothamsted experiments fortunately furnish us with a number of these valuable results. _Quantity of Nitrates formed in the soils of Fallow Fields._ These researches had to be carried out on soil taken from fields lying in bare fallow; for no true estimate of the amount of nitrates formed could have been obtained from _cropped_ fields. In the first 27 inches of soil of six separate fields, nitrate-nitrogen was found to vary from 36.3 lb. to 59.9 lb. per acre. In four of these fields the largest proportion was found in the first 9 inches of soil; in the remaining two, in the second 9 inches; while the third 9 inches in two fields showed almost as large a proportion as the first 9 inches.[121] _Position of Nitrates depends on Season._ The position of nitrates in the soil depends largely on the season; for, as has been already pointed out, their production is almost entirely limited to the surface-soil, and it is only by being washed down in rain that they find their way to the lower layers. A wet season, therefore, has the effect of increasing their percentage in the lower soil-layers. _Nitrates in Drainage-waters._