It may easily be understood, that the workers accustomed to respect their queen, whose presence is a real necessity to them, crowd after her; and the formation of the first swarm creates no difficulty in this respect. But you will undoubtedly ask, Sir, What motive can induce the workers to follow their queen from the hive, while they treat the young queens very ill, and, even in their most amicable moments, testify perfect indifference towards them. Probably it is to escape the heat to which the hive is then exposed. The extreme agitation of the females leads them to traverse the combs in all directions. They pass through groupes of bees, injure and derange them; they communicate a kind of delirium, and these tumultuous motions raise the temperature to an insupportable degree. We have frequently proved it by the thermometer. In a populous hive it commonly stands between 92 deg. and 97 deg., in a fine day of spring; but during the tumult which precedes swarming, it rises above 104 deg. And this is heat intolerable to bees. When exposed to it, they rush impetuously towards the outlets of the hive and depart. In general they cannot endure the sudden augmentation of heat, and in that case quit their dwelling; neither do those returning from the fields enter when the temperature is extraordinary. I am certain, from direct experiments, that the impetuous courses of the queen over the combs, actually throws the workers into agitation; and I was able to ascertain it in the following manner. I wished to avoid a complication of causes. It was particularly important to learn, whether the queen would impart her agitation but not at the time of swarming. Therefore I took two females still virgins, but capable of fecundation for above five days, and put one in a glass hive sufficiently populous; the other I put into a different hive of the same kind. Then I shut the hives in such a way that there was no possibility of their escape. The air had free circulation. I then prepared to observe the hives every moment that the fineness of the weather would invite both males and females to go abroad, for the purpose of fecundation. Next morning, the weather being gloomy, no male left the hive, and the bees were tranquil; but towards eleven of the following day, the sun shining bright, both queens began to run about seeking an exit from every part of their dwelling; and from their inability to find one, traversed the combs with the most evident symptoms of disquiet and agitation. The bees soon participated of the same disorder; they crowded towards that part of the hive where the openings were placed; unable to escape they ascended with equal rapidity, and ran heedlessly over the cells until four in the afternoon. It is nearly about this time that the sun declining in the horizon recalls the males; queens requiring fecundation never remain later abroad. The two females became calmer, and tranquillity was in a short time restored.