In experiments 6, 7, and 8, in which quantities of chloroform were employed intermediate between one and two grains to each hundred cubic inches of air, a moderate amount of insensibility was induced, corresponding very much with the state of patients during operations under chloroform. The experiments from 13 to 18 show that quantities of chloroform, exceeding two grains to 100 cubic inches of air, have a tendency to embarrass and arrest the function of respiration, if the inhalation is continued. I have not yet been able to determine satisfactorily the exact proportion of chloroform which requires to be absorbed to arrest the respiration of animals of warm blood. I believe there is a definite proportion which has this effect, but there are two reasons why it is not so easy to ascertain it, as to ascertain the proportion which causes the minor degrees of narcotism. In the first place, the breathing often becomes very feeble before it ceases, so that the animal inhales and absorbs but very little chloroform, and remains on the brink of dying for some time. In the next place, the temperature of the body falls in a deep state of narcotism, especially in small animals; and, as the temperature falls, the amount of chloroform which the blood can dissolve from any given mixture of air and vapour increases. Judging from the experiments numbered 14 to 18, three grains of chloroform to each hundred cubic inches of air must be very nearly the quantity which has the power of arresting the breathing when the temperature of the body is 100°; and as three grains of chloroform produce 2·3 cubic inches of vapour, and air at 100° is capable of taking up 43·3 per cent. of its volume, it follows that the blood must contain between 1–18th and 1–19th as much chloroform as it is capable of dissolving, at the time when the respiration is arrested. In the 14th experiment, the breathing of the two mice was on the point of being stopped by two and a half grains of chloroform in each hundred cubic inches of air, but during the thirteen minutes which the mice breathed the vapour, their temperature fell to about 90°. Air, when saturated with the vapour of chloroform at this temperature, contains 35 per cent., and two grains and a half of chloroform yield 1·917 cubic inches of vapour; so by a calculation similar to that made at page 68, the mice at the time when the breathing was about to cease must have absorbed 1–18th part as much chloroform as their circulating fluids were capable of dissolving. The reader will have observed that, in the experiments related above, the mice became much more quickly affected than the guineapigs and cats. The reason of this is their quicker respiration and circulation, and much more diminutive size. Little birds, such as linnets and sparrows, are also very quickly affected by chloroform. Frogs are more slowly affected, owing to their languid respiration, unless the vapour to which they are exposed is very strong.