The methods used in the experiments with vegetable purgatives were practically the same as those used by Loeb. The animal was bisected just above the ring of sense organs in order to entirely remove the margin containing the main nervous system. The center was then placed in mixtures of sea-water and solutions of the purgatives. The center never beats in pure sea-water, but was found to beat vigorously in sea-water to which a small quantity of a solution of cascara, rhubarb, aloin, podophyllin, or colocynth had been added. It was necessary to dissolve the cascara and rhubarb extracts in m/24 NaHCO_{3}, since they are not soluble in pure water. The centers do not beat in sea-water to which pure m/24 NaHCO_{3} has been added in quantities equivalent to those added with the purgative solution. A solution of ¹⁄₄ g. cascara extract was made in 50 c.c. m/24 NaHCO_{3}. It was found that a mixture of 25 c.c. sea-water + 2 c.c. of this cascara solution was the most favorable for producing rhythmical contractions in the isolated center of Polyorchis. Contractions lasted 10-15 minutes. A solution of rhubarb extract of the same strength was made. The optimal mixture in this case is 25 c.c. sea-water + 0.5 c.c. or 1 c.c. rhubarb solution. In this mixture the contractions develop quickly and last 15 minutes or more. With aloin the concentration of the purgative needed to produce optimal results was somewhat greater than in cascara or rhubarb. Colocynth and podophyllin act similarly, but the contractions soon cease. These vegetable purgatives thus act on the jellyfish, Polyorchis, in a way quite similar to that described by Loeb for saline purgatives. Pilocarpine, though not used as a purgative on account of its special action on other organs of the body, has a powerful action also on the intestine. Its influence on the intestine is much like that of barium chloride. It causes violent contractions of the musculature of the gut and very active peristaltic movements. This is the case in whatever way the substance is administered. A few drops of a ¹⁄₁₀% solution of pilocarpine hydrochlorate in distilled water poured on the serous surfaces of the rabbit’s intestine brings about almost immediately violent peristaltic movements. In addition to this there is an increase in the amount of fluid secreted into the intestine, 20-30 c.c. gathering in the small intestine in an hour. The evacuation of faeces takes place in about three-quarters of an hour. These may be of a semifluid character, and with larger doses resemble the faeces produced by BaCl_{2}. The antagonism between pilocarpine and CaCl_{2} is incomplete. CaCl_{2} is capable of inhibiting only temporarily the movements caused by pilocarpine.