On May 6, a second nitrogen balance was attempted covering a period of seven days, in which, as before, there was an exact comparison of the income and output of nitrogen. In this period of seven days, as shown in the accompanying tables, the fuel value of the food was essentially the same as in the preceding period, but the amount of proteid food was increased to an average intake of 10.10 grams per day. Under these conditions there was a distinct plus balance for the seven days amounting to 2.425 grams, thus showing that with this quantity of nitrogenous food the body was laying on nitrogen to the extent of 0.346 gram per day. The average daily amount of nitrogen metabolized during this period was only 8.18 grams, being quite noticeably below the average daily amount for the year. In other words, the results of this balance period show that with a consumption of food sufficient to yield about 2200 calories per day, the body of this subject needed to metabolize only 8.25 grams of nitrogen per day to more than maintain nitrogen equilibrium. Following are the tables of results: _Friday, May 6, 1904._ Breakfast.—Oatmeal 345 grams, butter 7 grams, sugar 30 grams, milk 100 grams, coffee 180 grams. Dinner.—Bread 67 grams, potato 71 grams, corn 179 grams, pie 133 grams, milk 200 grams. Supper.—Biscuit 75 grams, butter 11 grams, potato 106 grams, cake 52 grams, apricots 75 grams, milk 230 grams. Food. Grams. Per cent Nitrogen. Total Nitrogen. Oatmeal 345 × 0.40 = 1.380 grams. Butter 7 + 11 = 18 × 0.11 = 0.020 Sugar 30 × 0.00 = 0.000 Milk 100 + 200 + 230 = 530 × 0.54 = 2.862 Coffee 180 × 0.14 = 0.252 Bread 67 × 1.39 = 0.931 Potato (dinner) 71 × 0.52 = 0.369 Corn 179 × 0.44 = 0.788 Pie 133 × 0.54 = 0.718 Biscuit 75 × 1.21 = 0.908 Potato (supper) 106 × 0.36 = 0.382 Cake 52 × 0.90 = 0.468 Apricots 75 × 0.21 = 0.158 ------ Total nitrogen in food 9.236 grams. Total nitrogen in urine 8.720 Fuel value of the food 2080 calories. _Saturday, May 7, 1904._ Breakfast.—Oatmeal 382 grams, milk 100 grams, coffee 185 grams, butter 10 grams, sugar 30 grams. Dinner.—Bread 93 grams, potato 67 grams, rice pudding 141 grams, milk 200 grams. Supper.—Bread 67 grams, butter 13 grams, potato salad 122 grams, milk 240 grams, coffee 120 grams. Food. Grams. Per cent Nitrogen. Total Nitrogen.