It might be thought that out of its vessels, and exposed to the contact of the air, the blood might imbibe a pernicious principle, or be deprived of that which is requisite for the maintenance of life. It might be imagined, that to this cause was owing the sudden death of the dog, on the injection of the brain with venous blood. To shew that this was not the case, I made a small opening in the jugular of a dog, to which I adapted a moderately warm syringe, and pumped the blood immediately from the vein.--It was afterwards thrown into the carotid: the symptoms were the same as the preceding, but less marked, and the death of the creature induced more slowly.--It is probable, then, that the air when in contact with the living blood without its vessels may alter it a little, but the essential cause of death is still the same. Hence it appears that the black blood either is not an excitant capable of keeping up the cerebral action, or that it acts in a deleterious manner, upon the brain. The injection by the carotid of various other substances will produce analogous effects. I have killed animals in this way with ink, oil, wine, and water coloured with indigo. The greater number of the excrementitious fluids, such as urine, bile, the mucus of catarrhs, occasion death also by their simple presence on the brain. The serosity of the blood is fatal, but not as quickly so. Now it is certainly upon the substance of the brain, and not upon the internal surface of the arteries, that these different substances exert their influence. I have injected them all into the crural artery. In this way they are none of them mortal, but occasion always a torpor, amounting even to paralysis at times.[92] The black blood is doubtless fatal to the brain, the brain becoming at once a tonic from its presence. In what way does it act? I do not pretend to determine the manner; for this were only to begin a series of conjectures. By this time we are authorised to conclude, that in asphyxiæ, the circulation which continues for some time after the interruption of the chemical functions of the lungs, interrupts the cerebral functions, from its being composed of black blood only. The fact is proved in another manner, for the movements of the brain continue to be made as usual. If the cerebral mass be exposed, and the creature asphyxiated, the animal life will be extinguished, but the motion of the brain will be apparent still. Since then the latter cause of life subsists, the cause of death must be in the nature of the fluid, by which the organ is penetrated.