The electric machine was in action, and one of the attendants happening to approach or touch one of the frogs, the man as well as Madame Galvani observed that the limbs were violently agitated. Galvani was at once informed of this, and he made repeated experiments, which showed him that the convulsive movements only took place when a spark was drawn from the prime conductor of the electric machine, and while the nerve was touched by a conductor. Galvani then suspended a number of frogs to a railing by metal hooks, with a view to experiment upon them with atmospherical electricity. But the frogs’ limbs were again agitated when no electricity was apparent, and Galvani after some consideration came to the conclusion that the movement was owing to the position the animals assumed with reference to the metallic bodies. Thus when muscle and nerve were in contact with metallic bodies and connected by metal, the movements of the limbs were observable, and the greater the surface contact the greater was the convulsion. The philosopher next tried various metals, and discovered that the most powerful combination was zinc and silver. Galvani, in 1791, published his discovery and his theory that the body acted as a Leyden jar, different parts being in a different state of electricity. No sooner were his deductions published than all Europe was in a ferment, and philosophers of all nations were discussing it. Fowler, Valli, Robison, Wells, Humboldt, etc., all were deeply interested, but none of them appear to have arrived at so correct conclusions as did Volta, the physician of Pavia. “Wherever frogs were to be found,” says Du Bois Reymond, “and where two different kinds of metal could be procured, everybody was anxious to see the mangled limbs of frogs brought to life in this wonderful way. Physiologists believed that at length they should realize their visions of a vital power, and physicians thought no cure was impossible.” But notwithstanding the popular theory, Volta, in his letters to Carallo, while giving a full and clear account of the discovery made by Galvani and his own experiments, attacked and finally defeated the Professor. Volta quite upset Galvani’s Leyden-jar theory; Volta says that it was by accident that Mr. Galvani discovered the phenomenon, and by which he was more astonished than he ought to have been. Volta’s letters will be found in the _Philosophical Transactions_ of the Royal Society (in French), and he attributes the effect to the metals which produced a small amount of electricity. He found that the nerve was acted upon on even parts of a muscle laid upon two different metals, and if those were united, a contraction took place.