For drawing from the microscope the improved form of the _camera
lucida_, or the latest model of the _Edinger drawing-apparatus_ are
recommended. Both of these instruments have recently been greatly
improved. The _Zeiss microphotographic apparatus_ is by far the
best for microphotographic work. For the _polarization-microscope_,
_microspectroscope_, and the complicated and expensive _ultra-violet_
and _dark-field_ apparatus the worker is referred to the Zeiss
catalogues. A simple and practical _dark-field_ method for the
illumination of bacteria, spirochætes and ultramicroscopic particles
suspended in fluids requires only a strong illumination and the use
of a _Zeiss_, _Leitz_, or _Reichert dark-field condenser_; or the
very simple “India-ink” method may be used for the demonstration of
spirochætes. (See Staining of Spirochætes.) Especial instruments
for easily finding a certain field are obtainable, and are of great
convenience in marking slides for photographic purposes.
[Illustration: FIG. 51.—A good practical microtome for paraffin and
celloidin work. Well-adapted to needs of students and beginners.]
Next to the microscope the most important instrument in pathologic
work is the =microtome=. One that can be used for either celloidin or
paraffin work, and that can also be utilized as a freezing microtome,
should be selected in private work, when economy is desired. The
majority can be used for either paraffin or celloidin, and one of the
Becker models can be easily attached to the carbonic acid holder for
the cutting of frozen material. The Bardeen freezing microtome is
relatively inexpensive, very satisfactory, and can be easily attached
to the carbonic acid tanks. It can be recommended for freezing work.
The “slide” type, either that in which the knife-holder is moved by
the hand directly or by a crank turned by the hand, is advised for
ordinary diagnostic work. For students and beginners the crank is of
great advantage, as the knife is securely held and cannot jump. The
best microtomes are made by Schanze of Leipzig, Jung of Heidelberg,
Becker of Göttingen, the Cambridge Scientific Co., the International
Instrument Co., and the Bausch & Lomb Co. For either celloidin or
paraffin work the medium or large Schanze slide-microtome or the
Minot’s precision microtome are recommended; for cutting serial
sections in paraffin the latest modification of the Minot automatic
rotary microtome is especially adapted. The best =microtome knives=
are made by Walb of Heidelberg. A long, heavy knife is to be
preferred to a light one. For the freezing microtome a knife of the
type of the blade of a carpenter’s plane set in a wooden handle
should be used, Hones and strops of the best quality are necessary.
Public-domain text, read in full here on John Shaqi.
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