To prepare _serial-sections_ from celloidin blocks the sections as
they are cut must be arranged in their order on the knife-blade and
thence transferred in this order to a slide or glass-plate, to which
they are either fastened so that they cannot become loose during
the staining process, or they are fastened together by a film of
celloidin and stained in one piece. To fasten the celloidin sections
to the slide _Mallory_ and _Wright_ suggest the cutting of the block
in 95 per cent alcohol, and the arrangement of the sections in their
order on the knife, whence they are drawn on to a dry, clean and
numbered slide. Ether vapor from a half-full bottle of ether is
then poured over the slide, slowly, to flatten out the sections and
fasten down the frilled edges. The slides are then transferred to
80 per cent alcohol to harden the celloidin, and they are kept in
this solution until ready for staining. Celloidin sections may also
be fastened to the slide by albumin glycerin (equal parts white of
egg and glycerin with a crystal of phenol or thymol). The celloidin
sections, as they are cut, may be transferred to a dry gelatinized
slide or plate (16 grms. gelatin in 300 cc. warm water), and are
then covered with a thin celloidin; the slide is then placed in
water at 50°C., the gelatin dissolves and the celloidin film floats
off; the latter is then stained as one section. Serial sections of
celloidin-blocks may also be arranged upon slides or plates covered
with a coating of the _Schmorl-Obregia_ sugar-dextrin solution or
diluted New Orleans black molasses; the slide or plate is flooded
with absolute alcohol; drained; a thin celloidin is then poured over
it; it is immersed in warm water and the celloidin-sheet containing
the sections is liberated, and is then stained as a whole or cut
into strips as desired. The molasses method is the cheapest and
simplest method, and much to be preferred to the Weigert closet-paper
method, by which the sections are arranged upon a strip of moist
closet-paper which is either held upon the slide or kept upon a
piece of blotting-paper wet with 80 per cent alcohol. A slide or
glass-plate is covered with a thin layer of celloidin and the strip
of paper containing the sections is laid upon the celloidin surface,
the sections down, so that these stick to the slide as the paper is
carefully peeled off. The preparation is dried with filter-paper and
a thin layer of celloidin poured over the slide and sections. The
celloidin is then hardened in 80 per cent alcohol and the sections
stained on the slide; or the celloidin film is removed by immersion
in warm water, and then treated as one section. Celloidin films may
be marked with a brush dipped in a water solution of methylene-blue.
This should be done as soon as they are made. _Langhans’ method_
is advised for the remounting of serial sections from tissues
stained in bulk and imbedded in celloidin. The sections are cut in
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