There is but one disadvantage in the case of
carbol-xylol; some of the phenols in the market cause a fading of
eosin and hæmatoxylin stains. _DeWitt_ has shown that this fault
can be corrected by redistillation, stopping the distillation as
soon as the temperature begins to rise above the constant boiling
point of the phenol; or the carbol-xylol that fades the stains can
also be corrected by supersaturating it with a mixture of sodium
bicarbonate one part, and sodium-potassium tartrate two parts.
Sections kept in xylol or carbol-xylol should be protected from
dust and evaporation; it is not a good plan to keep them in these
solutions for more than 24 hours.
VI. MOUNTING.
Permanent mounts are made in glycerin, potassium acetate, lævulose,
glycerin gelatin, balsam, damar or colophonium. For celloidin and
paraffin sections a solution of =Canada balsam= in xylol is most
commonly used for mounting. Celloidin sections (celloidin films are
best cut into strips and single sections when in the carbol-xylol;
the wheel-shaped paper-cutter used by paper-hangers is the best
instrument for this purpose) are lifted onto the slide from the
clearing-fluid; folds or wrinkles in the celloidin are straightened
or removed by cutting the celloidin at right angles to the section
in order to relieve the tension, and the section is then blotted
firmly against the slide by means of a pad of absorbent paper.
The greatest care should be taken to prevent wrinkling, folding
or turning over of the edge of the section. As soon as the pad
is removed a drop of balsam is placed upon the section and the
cover-glass put over it. There should be just enough balsam used to
fill the space between cover-slip and slide, so that air-bubbles
are not formed. The balsam must not be so thin that the cover-glass
will float about on the liquid, or so thick that it does not spread
well. In the latter case warming the slide may cause it to spread
more readily, but care must be taken not to injure the stain by
over-heating. Paraffin sections on the slide are similarly blotted
and covered with balsam and cover-glass; those on cover-slips are
blotted between folds of absorbent paper and immediately placed
with section-side downward upon a drop of balsam that has been put
upon the slide.
=Xylol-damar= may be used in place of Canada balsam; it is cheaper
and colorless, but it tends to become cloudy. =Colophonium= is
the cheapest of the three and has but little color; it is highly
recommended by many workers. In a xylol-solution it may be used for
aniline stains; a chloroform solution is advisable for the mounting
of osmic-acid preparations; while a solution in turpentine and
shellac is recommended for Weigert’s neuroglia method, Wright’s
blood-stain, and other special staining methods.
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