4. Wash, dehydrate and clear quickly, as for eosin. (Dry picric acid may be added to the carbol-xylol.) 5. Mount in balsam. c. =Haematoxylin and Acid Fuchsin.= Stain with hæmatoxylin, and after washing use a 1 per cent water solution of acid fuchsin, until section is sufficiently red; wash; dehydrate; clear; mount. d. =Haematoxylin and Orange G.= Stain with hæmatoxylin, and after washing use a 1 per cent water solution of orange G, staining ¼-3 hours. Treat otherwise as for eosin-staining. e. =Carmine and Picric Acid.= Stain with borax- or lithium-carmine; differentiate in acid alcohol and wash thoroughly. Then counterstain with picric acid, as for hæmatoxylin and picric acid. Carmine and picric acid may also be combined in one stain, as picro-carmine, but this is rarely used at the present. f. =Eosin and Methylene-blue.= (For tissues fixed in mercuric chloride or Zenker’s.) 1. Stain in a 5-10 per cent aqueous eosin for 20 minutes or longer, until a deep eosin-stain is obtained. 2. Wash out excess of eosin in water. 3. Stain in Unna’s alkaline methylene-blue, diluted 1-4 or 5 with water, 10-15 minutes. 4. Wash in water. 5. Differentiate in 95 per cent alcohol, keeping the section in constant motion to obtain a uniform decolorization. Control process under microscope. _Wolbach_ advises the use of a 0.75-1.5 per cent solution of colophonium in methyl alcohol as a differentiating medium instead of 95 per cent alcohol. For tissues fixed in formol or alcohol a 10 per cent solution should be used. 6. When the background is pink, dehydrate quickly with absolute alcohol, or in 95 per cent by blotting on slide with xylol, until clear. 7. Clear in xylol. 8. Mount in balsam. g. =Van Gieson’s Method.= 1. Stain in Weigert’s iron-hæmatoxylin, or overstain if any other hæmatoxylin is used. (Weigert’s gives the best results, as it does not decolorize so readily.) 2. Wash thoroughly. 3. Stain in Van Gieson’s mixture (acid fuchsin 1.5 grms., saturated water solution of picric acid [0.6 per cent] 150 cc. This mixture keeps well. Add 1 cc. of this stock solution to 10 cc. of saturated water solution of picric acid. Stain in this for 10 seconds). 4. Wash quickly; dehydrate in alcohol; clear in xylol or carbol-xylol; mount in balsam.