2. =Best’s Carmine Method for Glycogen.= 1. Fix in absolute alcohol; imbed in celloidin; cut. 2. Stain in hæmatoxylin; differentiate in acid alcohol. 3. Wash in water. 4. Stain in filtered carmine mixture (carmine 1 grm., ammonium chlorate 2 grms., lithium carbonate O.5 grm., water 50 cc.; bring to boiling point, and when cool, add 20 cc. of strong liquid ammonia. Keep in dark; can be used after 2-3 days and gives good results up to 14 days) 2 parts, strong ammonia 3 parts, methyl alcohol 6 parts. Make fresh each time it is used, as it soon precipitates; do not filter; stain few sections at a time ¾-1 hour. 5. Differentiate 1-2 minutes in a mixture of absolute alcohol 4 parts, methyl alcohol 2 parts, water 5 parts. 6. Wash in 80 per cent alcohol. 7. Dehydrate in absolute alcohol. 8. Clear in xylol; mount in balsam. Glycogen is stained red; nuclei blue; dense connective-tissue, mast-cell granules, protoplasm of gastric glands, etc., red; but these can all be distinguished morphologically from glycogen. This is by far the best method for the staining of glycogen. =XII. HYALIN.= Epithelial hyalin (colloid) stains red or violet with hæmatoxylin and eosin; it takes the other acid dyes and stains to some degree with basic aniline stains. Van Gieson’s stains it a yellow, orange or brownish-pink. Kresyl-echt-violett gives it a deep indigo-blue color or a more green robin-egg blue. _Connective-tissue hyalin_ stains deep brilliant red with Van Gieson’s; this is the best method for differentiating connective-tissue hyalin from amyloid or epithelial hyalin. Russell’s method also stains hyalin red. =XIII. HYDROPIC DEGENERATION.= Fix by heat or formol-alcohol. Imbed in celloidin; stain with hæmatoxylin and eosin. =XIV. HYPERTROPHY.= Fix in Müller’s or mercuric chloride for simple staining; for study of nuclei fix in Flemming’s and stain with safranin. =XV. INFLAMMATION.= The process of inflammation may be studied to advantage in the mesentery, web or tongue of the curarized living frog, by stretching these parts over a cork-ring attached to a glass plate on which the animal rests. The exposed tissues must be kept moist with physiologic salt solution. Heat, chemicals or other irritants may be employed to produce the inflammatory reaction. For the study of the inflammatory process in sections the ordinary fixations may be employed, but for the study of the nuclei, mitotic figures and cell-granulations fixation in Flemming’s, Zenker’s, etc., is advised. Safranin, methylene blue and eosin, the various stains used in the study of blood-cells, etc., may be used. 1. =Staining of Mast-cells.= _a. Kresyl-echt-violett_ used as for amyloid or mucin is the best stain for mast-cells. The cell-granules stain bright rose-red. _b. Ehrlich’s Dahlia Method._