6. =Micrococcus Tetragenus.= Stain with Gram’s or watery solutions
of basic aniline dyes.
_b. Bacilli._
1. =Anthrax-bacillus.= Stain with Gram-Weigert’s and contrast with
Bismarck brown or lithium-carmine. Stains also with strong watery
gentian-violet solution, with differentiation in strong alcohol.
2. =Bacillus of Malignant Oedema.= Gram-negative. Stain with watery
solution or gentian-violet.
3. =Bacillus of Tetanus.= Gram-positive. Stains with watery
solutions of basic aniline dyes.
4. =Bacillus Aërogenes Capsulatus.= Gram-positive. Stains with
other aniline stains.
5. =Bacillus Pyocyaneus.= Stains with Gram’s and other aniline dyes.
6. =Bacillus of Influenza.= Gram-negative. Fix tissue in alcohol.
Stain with dilute carbol-fuchsin and differentiate in dilute acetic
acid.
7. =Koch-Week’s Bacillus.= Gram-negative.
8. =Bacillus of Bubonic Plague.= Gram-negative. Stain by
_Gaffky’s method_ (Fix in alcohol or a mixture of glacial acetic
acid 10.0, chloroform 30.0, and 96 per cent alcohol 60.0, imbed
in paraffin, stain 2-3 hours in weak watery methylene-blue,
dehydrate quickly in absolute alcohol, xylol, balsam). It may
also be stained by 24 hours in concentrated solution of fuchsin
in glycerin, rapid differentiation in weak acetic; alcohol;
xylol; balsam. Alcohol or mercuric chloride fixation should be
used, as formol fixation does not give good staining.
9. =Typhoid Bacillus.= Löffler’s methylene-blue or carbol-fuchsin,
staining 24 hours, decolorizing in dilute acetic and washing
rapidly in alcohol. Zieler’s method may also be used. It is
Gram-negative.
10. =Paratyphoid Bacillus.= Stains like the typhoid bacillus.
11. =Colon Bacillus.= Gram-negative. May be stained with Löffler’s
methylene-blue or carbol-fuchsin.
12. =Diphtheria Bacillus.= May be stained in sections of
diphtheritic membranes with Löffler’s methylene-blue, watery
aniline stains, or with Gram’s if the decolorization is not carried
too far.
13. =Bacillus of Chancroid.= Gram-negative. Stain according to
_Unna’s method_:—
1. Fix in alcohol.
2. Stain 5-10 minutes in a mixture of a solution of
methylene-blue 1 grm., potassium carbonate 1 grm., alcohol 20
cc., water 100 cc., and a solution of methylene-blue 1 grm.,
borax 1 grm., water 100 cc.
3. Place sections on slide; blot.
4. Decolorize in Unna’s glycerin-ether mixture.
5. Dry; dehydrate in alcohol.
6. Xylol; balsam.
14. =Bacillus of Glanders.= Gram-negative. Stain with Zieler’s
method or with Löffler’s methylene-blue, differentiating in weak
acetic.
_Noniewicz’s Method._
1. Stain with Löffler’s methylene-blue 2-5 minutes.
2. Wash in water.
3. Differentiate for about 5 seconds in a mixture of ½ per cent
acetic acid 75 cc., ½ per cent watery solution tropaeolin 25 cc.
4. Wash in water; dry by blotting; xylol; balsam.
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