4. =MODIFICATIONS OF THE ROMANOWSKY METHOD.= A large group of stains has resulted from various applications of the Romanowsky idea of uniting equimolecular proportions of methylene-blue and eosin, and the solution of the dyes so obtained in some suitable solvent. These dyes consist of mixtures of methylene violet, methylene azure, eosinate of methylene blue, etc., and can be obtained from Grübler and Co. under various names, such as _Azur-blau_, _Bleu Borrel_, _Giemsa’s stain_, _Leishman’s stain_, etc. _Hastings_, _Leishman_, _Wright_ and others have combined the Romanowsky method with that of Jenner by dissolving the new dyes obtained by their various modifications in pure methyl alcohol, so as to form a solution that will fix and stain at the same time. _Hastings’ stain_ is a modification of _Nocht’s stain_; _Wright’s stain_ is a modification of the _Leishman-Romanowsky_ method. The revised directions given by _Wright_ for making and using his stain are here given. Wright’s method and the Giemsa stain possess all of the staining advantages afforded by the variations of the Romanowsky method, and are alone given here. The former is recommended for blood-work, the latter for the staining of protozoa. Wright’s Blood-stain. To a 0.5 per cent aqueous solution of sodium bicarbonate add methylene-blue (B.X or medicinal) in the proportion of 1 grm. of the dye to each 100 cc. of the solution. Heat the mixture in a steam sterilizer at 100°C. for one full hour, counting the time after the sterilizer has become thoroughly heated. The mixture should be placed in a flask of such size and shape that the fluid will not be more than 6 cm. deep. After heating, allow the mixture to cool, placing the flask in cold water if desired, and then filter it to remove the precipitate. When cold the fluid should have a deep purple-red color when viewed in a thin layer by transmitted yellowish artificial light. It does not show this color while warm. To each 100 cc. of the filtered mixture add 500 cc. of a 0.1 per cent aqueous solution of yellow water-soluble eosin, and mix thoroughly. Collect on a filter the abundant precipitate which immediately appears. When the precipitate is dry, dissolve it in pure methyl alcohol (Merck’s) in the proportion of 0.1 grm. to 60 cc. of the alcohol. To facilitate solution the precipitate is to be rubbed up in a porcelain dish or mortar with a spatula or pestle. This alcoholic solution is the staining solution. It should be kept in a tightly-stoppered bottle. Should it become concentrated through evaporation methyl alcohol in proper quantity should be added. 1. Cover film with a given quantity of staining fluid by means of a medicine dropper.