Use any of the above methods for the staining of myelin sheaths, ganglion cells, axis cylinders, etc. Van Gieson’s is good for the demonstration of connective-tissue increase. For the demonstration of peripheral fibrils and nerve-endings consult textbooks on histology for Golgi methods, Ehrlich’s vital methylene-blue method, and the modifications of May, Drasch, and others. Platner’s Method. 1. Harden in 25 per cent solution of liq. ferri sesquichlor., 1-5 days. 2. Wash in water, until the addition of KCNS to the water yields no reaction. 3. Place in 75 per cent alcohol containing an excess of di-nitroresorcin, 2-30 days, according to the size of the piece of tissue. 4. Dehydrate in absolute alcohol. 5. Imbed; cut; dehydrate; clear; mount. Axis cylinders, emerald green. A good method for the rapid demonstration of pathological processes connected with the peripheral nerves. 9. =CHROMAFFINIC TISSUES.= Wiesel’s Method. 1. Fix 1-4 days in 10 parts of a 5 per cent potassium bichromate solution, 20 parts of 10 per cent formol, 20 parts distilled water. 2. 1-2 days in 5 per cent potassium bichromate. 3. Wash thoroughly in running water, 24 hours; harden in graded alcohols; imbed in paraffin. 4. Stain sections in a 1 per cent aqueous toluidin-blue or water-blue solution. 5. 5 minutes in tap-water. 6. Stain 20 minutes in a 1 per cent watery safranin solution. 7. 96 per cent, and then absolute alcohol, until the blue color appears. 8. Xylol; balsam. Chromaffinic cells green; other cells light blue, nuclei red. X. SKIN. Skin should be fixed in formol-Müller’s or formol, and should not be left too long in alcohol or xylol. Imbed in celloidin preferably. For general use the ordinary stains suffice; for the study of pigment stain with alum or lithium-carmine. Use Weigert’s elastic-tissue stain for the demonstration of elastic fibrils. The intercellular bridges may be demonstrated by Van Gieson’s (remaining unstained), or by various special staining methods. Herxheimer’s Method for Epithelial Fibrillae. 1. Harden; imbed; cut. 2. Stain in a saturated watery solution of kresyl-echt-violett. 3. Dehydrate in absolute alcohol; clear in clove oil; balsam. CHAPTER XXIX. MICROSCOPIC EXAMINATIONS FOR MEDICOLEGAL PURPOSES.