The purpose of placing sand at the bottom of each lysimeter is to offer a porous stratum in which free water may collect and rise to the level of the perforated copper tube, which would prevent any further rise by conveying the surplus above into the vault as drainage water. The soil above the tube will therefore be constantly drained and the sand below constantly saturated, unless the water be drawn up by the capillary action of the soil as the result of evaporation from the surface. By means of a proper arrangement within the vault, of a kind of Mariotte’s bottle, the water may be caused to flow back through the drainage tube into the lysimeter to take the place of that lost by evaporation, and thus maintain the level of free water just below the drainage tube. The water flowing back to the lysimeter, and the amount of drainage water, are carefully measured by a system of graduated tubes. The lysimeters thus constructed represent tile-drained land; in one case the tile being three feet below the surface and in the other six feet below. The drainage waters collected in the receiving bottles can be measured and analyzed from time to time, as occasion may require, to determine the amount of plant food which is removed. =179. Improved Method of Deherain.=[120]—Deherain’s earlier experiments were made in pots containing about sixty kilos of soil. These vases serve very well for some kinds of plants, but there are other kinds which do not grow at all normally when their roots are imprisoned. For instance, in pots, even of the largest size, wheat is always poor, beets irregular, maize never acquires its full development, and the conclusions which can be drawn from the experiments can not be predicated of the action of the plant under conditions entirely normal. It is necessary therefore to carry on the work in an entirely different way, and to construct boxes so large as to make the conditions of growth entirely normal. The arrangement of these boxes is shown in Fig. 24. They are placed in a large trench, two meters wide, one meter deep, and forty meters long. There are twenty boxes in this trench, the upper surface of each containing four square meters area. The boxes are one meter deep, and therefore can contain four cubic meters of soil. The sides and bottoms of the boxes are made of iron lattice work, covered with a cement which renders them impervious to water.