(2) Sunshine, or the proximity of any other source of heat, must be carefully excluded. The currents formed when the instrument is exposed to sunshine will vitiate the results. (3) The Mariotte’s bottle should be frequently cleansed, and the water used be as free from foreign matters as possible. For ordinary purposes it is scarcely necessary to use distilled water. The quantities used are so large as to render it difficult to maintain an adequate supply, and the errors resulting from the use of any water fit for drinking purposes are too slight to be perceptible, so long as no considerable development of the animal and vegetable germs is allowed. Water containing the slimy filaments of fungoid growths and moss protonema, algae, vorticellae, etc., will not only cause errors by obstructing the stop-cock at low velocities, but these organisms will cause a coalescence of sediments that defies any ordinary churning, and completely vitiates the operation. (4) The amount of sediment discharged at any time must not exceed that producing a moderate turbidity. Whenever the discharge becomes so copious as to render the moving column opaque, the sediments assume a mixed character, coarse grains being, apparently, upborne by the multitude of light ones whose hydraulic value lies considerably below the velocity used, while the churner also fails to resolve the molecular aggregates which must be perpetually reforming where contact is so close and frequent. This difficulty is especially apt to occur when too large a quantity of material has been used for analysis, or when one sediment constitutes an unusually large portion of it. Within certain limits the smaller the quantity employed the more concordant are the results. Between ten and fifteen grams is the proper amount for an instrument of the dimensions given above. =224. Preparation of the Sample.=[153]—In some cases simple sifting will be sufficient to prepare the air-dried soil for the elutriator. In most cases, however, some mechanical aid must be invoked to secure particles of sufficient fineness. Nothing harder than a rubber pestle should be used and care must be taken not to break up any calcareous or ferruginous masses which the particles of fine soil may contain. The use of water in this mechanical attrition should be avoided, if possible, but in some heavy clay and adobe soils wetting becomes necessary. In this case the parts separated by the sieve are collected separately and the turbid mass removed by water and dried for further examination. A sieve of 0.5 millimeter mesh is recommended as the best because that is almost exactly the diameter of the particles passing off at the maximum velocity of sixty-four millimeters per second to which the elutriator is adapted. The particles passing the 0.5 millimeter mesh are called fine earth.