The method has also been applied to the mixture of ammoniacal and organic nitrogen and to the mixture of ammoniacal, nitric, and organic nitrogen, the combustions having been made both in an iron and a glass tube. The amounts of material to be used vary from one-half gram to a gram, according to its richness in nitrogen. The combustion should be terminated in forty minutes. When a combustion is terminated, the acid containing the ammonia is placed in a beaker and boiled for two or three minutes to drive off the sulfurous and carbonic acids. The titration is then conducted in the usual manner. The combustion can be carried on just as well in an iron tube as in a glass one. The reagents employed, especially soda-lime, being hygroscopic, a little water is disengaged in heating, which is condensed at the cold extremity of the tube, and which may absorb a little ammonia, unless special precautions are taken to have the materials dry. THE MOIST COMBUSTION PROCESS. =178. Historical.=—As long ago as 1868 Wanklyn proposed to conduct the combustion of organic bodies in a wet way, using potassium permanganate as the oxidizing body.[147] About ten years after this he attempted to extend the method so as to estimate the quantity of proteid matter in a sample by treatment with an alkaline solution in presence of the permanganate salt. One gram of the finely pulverized sample was treated in a liter flask with one-tenth normal potash lye. After digestion for some time, from ten to twenty cubic centimeters were taken for the determination. According to the supposition of Wanklyn, pure albuminoid matters thus treated yielded one-tenth of their weight of ammonia, or about fifty per cent of the total nitrogen appeared as ammonia. The ammonia content of the sample was determined by the colorimetric process devised by Nessler. It is needless to add that the process of Wanklyn proved to be of no practical use whatever, acting differently on different albuminoid matters, and even on the same substance. No other attempt was made to perfect the moist combustion process until Kjeldahl[148] introduced the sulfuric acid method in 1883. The simplicity, economy, and adaptability of this method have brought it into general use. At first the process was only applied to organic nitrogenous compounds in the absence of nitrates, but especially by the modifications proposed by Asboth, Jodlbaur, and Scovell, it has been made applicable to all cases, with the possible exception of a few alkaloidal and allied bodies. The moist combustion process for determining nitrogen is now generally employed by chemists in all countries, not only for fertilizer control, but also for general work.