The process carried out as directed, when tested against pure sugar,
gives good results, not varying from the actual content of the sugar
by more than one-tenth per cent below or three-tenths above the true
content. The distinct pink coloration imparted to the solution by the
permanganate solution as soon as the iron is all oxidized to the ferric
state marks sharply the end of the reaction. In this respect this
process is very much to be preferred to the usual volumetric processes
depending upon the coloration produced with potassium ferrocyanid
by a copper salt for distinguishing the end of the reaction. It is
less convenient than the ordinary volumetric process by reason of the
somewhat tedious method of washing the precipitated cuprous oxid. When
a large number of analyses is to be made, however, the whole can be
washed with no more expenditure of time than is required for a single
sample. One analyst can, in this way, easily attend to fifty or a
hundred determinations at a time.
In the application of the permanganate method to the analysis of the
juices of sugar cane and sorghum it is directed to take 100 cubic
centimeters of the expressed juice and clarify by the addition of
twenty-five cubic centimeters of basic lead acetate, diluted with
water, containing enough of the lead acetate, however, to produce a
complete clarification. It is not necessary to remove the excess of
lead from the filtrate before the determination. Ten cubic centimeters
of the filtrate correspond to eight cubic centimeters of the original
juice. For percentage calculation the specific gravity of the original
juice must be known. Before the addition of the alkaline copper
solution, from fifty to seventy-five cubic centimeters of water should
be added to the clarified sugar juice and the amount of fehling
solution used in each case should be from fifty to seventy-five cubic
centimeters. The heating at 75° should be continued for half an hour
in order to insure complete reduction and oxidation of the sugar. The
sucrose can also be estimated in the same juices by inverting five
cubic centimeters of the clarified juice with five cubic centimeters
of dilute hydrochloric acid, by heating for an hour at a temperature
not above 90°. Before adding the acid for inversion, about 100 cubic
centimeters of water should be poured over the five cubic centimeters
of sugar solution. The washing of the suboxid and the estimation of the
amount reduced are accomplished in the manner above described.
This method has been extensively used in this laboratory and with very
satisfactory results. The only practical objection which can be urged
to it is in the time required for filtering. This fault is easily
remedied by adopting the method of filtering through asbestos felt
described in the next paragraph.
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