to be of considerable practical value to analysts, as it may be applied
for the qualitive detection of sucrose in seeds and other vegetable
products.[133]
=168. The Dextrose Group.=—In case the carbohydrate in question shows a
right-handed rotation and the absence of sucrose is established by the
polariscopic observation described above, the presence of the dextrose
group may be determined by the following test.[134]
Five grams of the carbohydrate are oxidized by boiling with from twenty
to thirty cubic centimeters of nitric acid of 1.15 specific gravity,
and then at gentle heat evaporated to dryness with stirring. If much
mucic acid be present, as will be the case if the original matter
contained lactose some water is added and the mixture well stirred,
and again evaporated to dryness to expel all nitric acid. The residue
should be of a brown color. The mass is again mixed with a little
water and the acid reaction neutralized by rubbing with fine-ground
potassium carbonate. The carbonate should be added in slight excess and
acetic acid added to the alkaline mixture, which is concentrated by
evaporation and allowed to stand a few days. At the end of this time
potassium saccharate has formed and is separated from the mother liquid
by pouring on a porous porcelain plate. The residue is collected,
dissolved in a little water and again allowed to crystallize, when it
is collected on a porous plate, as before, and washed by means of an
atomizer with a little aqueous spray until it is pure white and free of
any oxalic acid. The residual acid potassium saccharate may be weighed
after drying and then converted into the silver salt. The potash salt
for this purpose is dissolved in water, neutralized with ammonia and
precipitated with a solution of silver nitrate. The precipitate is
well stirred, collected on a gooch and washed and dried in a dark
place. It contains 50.94 percent of silver. All sugars which contain
the dextrose group yield silver saccharate when treated as above
described. Inulin, sorbose, arabinose and galactose yield no saccharic
acid under this treatment, and thus it is shown that they contain
no dextrose group. Milk sugar, maltose, the dextrins, raffinose and
sucrose yield saccharic acid when treated as above and therefore all
contain the dextrose group.
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