The hydrates of albumen have rotation powers which vary from [_a_]_{D}
= -71°.40 to [_a_]_{D} = -79°-05. From the chaotic state of knowledge
concerning the specific rotating power of the various albumens, it is
impossible to assign any number which will bear the test of criticism.
For the present, however, this number may be fixed at [_a_]_{D} = -70°
for the albumens which remain in solution in the liquids polarized for
milk sugar.[200]
Many reagents have been prepared for the removal of the disturbing
bodies from milk in order to make its polarization possible. Among
the precipitants which have been used in this laboratory may be
mentioned:[201]
(1) Saturated solution basic lead acetate, specific gravity 1.97:
(2) Nitric acid solution of mercuric nitrate diluted with an equal
volume of water: (=88.=)
(3) Acetic acid, specific gravity 1.040, containing twenty-nine per
cent acetic acid:
(4) Nitric acid, specific gravity 1.197, containing thirty per cent
nitric acid:
(5) Sulfuric acid, specific gravity 1.255, containing thirty-one per
cent sulfuric acid:
(6) Saturated solution of sodium chlorid:
(7) Saturated solution of magnesium sulfate:
(8) Solution of mercuric iodid in acetic acid, formula; potassium
iodid, 33.2 grams; mercuric chlorid, 13.5 grams; strong acetic acid,
20.0 cubic centimeters; water 640 cubic centimeters.
Alcohol, ether, and many solutions of mineral salts, hydrochloric and
other acids are also used as precipitants for albumen, but none of them
presents any advantages.
Experience has shown that the best results in polariscopic work are
secured by the use of either the mercuric iodid or the acid mercuric
nitrate for clarifying the milk. The latter reagent should be used
in quantities of about three cubic centimeters for each 100 of milk.
It is evident when it is desired to determine the residual nitrogen
in solution, the former reagent must be employed. The quantity of
albuminoid matter left in solution after clarification with mercurial
salts is so minute as to exert no sensible effect on the rotation of
the plane of polarized light produced by the lactose.
For purposes of calculation the gyrodynat of lactose in the ordinary
conditions of temperature and concentration may be represented by
[_a_]_{D} = 52°.5 (=107=).
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