=94. Calculation of Results.=—The percentage of sucrose in a solution
which has been polarized before and after inversion is calculated by an
appropriate formula from the data obtained or is taken directly from
tables. These tables are too long to insert here, and in point of fact
the calculation can be made from the formula almost as quickly as the
result can be taken from a table.
Two factors are commonly used in the calculations, one based on
the supposition that a sugar solution polarizing 100° to the right
will, after inversion, give a reading of 44° to the left, at zero
temperature. In the second formula in common use the polarization to
the left in the circumstances mentioned above is assumed to be 42.4, a
number reached by Landolt after a long series of experiments.[56] The
principle of the calculation of the percentage of sucrose is based upon
the original observation of Clerget to the effect that the algebraic
difference of the two readings, divided by 144, less half of the
temperature, will give the percentage of sucrose desired. The formula
by which this is obtained is
_a_ - _b_
_S_ = -----------.
_K_ - _t_
----
2
In this formula _a_ is the polarization on the sugar scale before
inversion, _b_ the polarization after inversion, _K_ the constant
representing the algebraic difference of the two polarizations of
pure sugar at 0° and _t_ the temperature of the observation. To _K_
may be assigned the values 144 or 142.4, the one in more common use.
In case the polarization, after inversion, is to the left, which is
more commonly the case, the sum of the two readings is taken for
_a_ - (-_b_) = _a_ + _b_; when both polarizations are to the right
or left the difference is taken. S is the percentage of sucrose desired.
_Example.—_Let the polarization before inversion be +95
and after inversion -26
and the temperature 20°
95 + 26
Then _S_= -------- = 121 ÷ 134 = 90.6.
144 - 10
Substituting the value 142.4 for _K_, the result of the calculation is
91.4.
In high grade sugars, therefore, the difference in the results secured
by taking the two values of _K_ amounts to about 1 per cent of sucrose.
For a further discussion of the theory and practice of inversion the
reader is referred to the articles of Herles, Herzfeld, and Wohl.[57]
=95. Method Of Lindet.=—Courtonne recommends the method of Lindet for
securing the inversion instead of the method of Clerget.[58] Modified
by Courtonne, the method is as follows:
Make two or three times the normal weight of sugar dissolved in water
to a volume of 200 or 300 cubic centimeters, as the case may be. After
thoroughly mixing proceed as follows:
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