There are two breeding seasons, one about April and the other about
September, and a winter hibernation. Not only animals brought in from
outside, but their offspring reared in domestication maintain these
normal habits in confinement, if the temperature does not exceed 17° C.
(pp. 499 and 534).
If, however, the temperature be artificially raised and kept at 25-30°
C., the males do not attach the eggs to themselves when spawning occurs
on land but let them lie. The adhesion of the eggs is said to be
hindered by the comparatively rapid drying of their surfaces.
More usually in the high temperatures the animals _take to the water_
and copulate there. The eggs are ejected into the water, and as their
gelatinous coverings immediately swell up, they do not stick to the
males.
The offspring thus derived from the parents subjected to heat for one
breeding-period only, whether they were laid in water or on land, did
not show departures from the normal type.
Kammerer states next, however, that in subsequent breeding-periods the
same parents frequently take to the water to breed, though they have
become quite accustomed to the heated chamber; and furthermore that if
such animals, having thus lost their instinct to brood their young, be
transferred to ordinary temperatures they do not readily reassume their
normal habits, but for several breeding seasons--at least four--will
take to the water. These parents lay from 90 to 115 eggs, which are
small and contain little yolk, and the larvae, on hatching, breathe with
their embryonic gills until they are absorbed instead of being broken
off as normally.
The offspring thus abnormally developed when they mature are said never
to brood their eggs. If they are derived from the earlier spawnings of
their parents, before, that is to say, the parents had been submitted
to the changed conditions long enough to transmit their effects, they
lay on land; but if they are derived from the later spawnings, they
lay in the water. These changes of habit are manifested without the
continued application of the abnormal experimental conditions, and, as I
understand the account, in normal conditions of temperature.
If the abnormal experimental conditions are continued, the toads always
lay in water, and their eggs become progressively smaller and more
numerous. The larvae in the fourth generation acquire three pairs of
gills instead of one pair, and are in other respects also different from
the normal form.
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