Although the discovery of the Throgmorton plot (Nov. 1583), and the consequent expulsion of the Spanish ambassador, Mendoza, were not immediately followed by open war between England and Spain, yet the course of events thenceforward tended directly to that issue. Elizabeth immediately proposed to the Dutch States to form a naval alliance against Spain, and to concert other measures for mutual defence. Orange met the offer with alacrity, and pressed Elizabeth to accept the sovereignty of Holland, Zealand, and Utrecht. Perhaps there was no former ruler of England who would not have clutched at such an opportunity of territorial aggrandisement. For Elizabeth it had no charms. Every sensible person now will applaud the sobriety of her aims. But though she eschewed territory, she desired to have military occupation of one or more coast fortresses, at all events for a time, both as a security for the fidelity of the Dutch to any engagements they might make with her, and to enable her to treat on more equal terms with France or Spain, if the Netherlands were destined, after all, to fall into the hands of one of those powers. While these negotiations were in progress, William of Orange was murdered (June 30/July 10, 1584). Alençon had died a month earlier. The sovereignty of the revolted Netherlands was thus vacant. Elizabeth advised a joint protectorate by France and England. But the Dutch had small confidence in protectorates, especially of the joint kind. What they wanted was a sovereign, and as Elizabeth would not accept them as her subjects they offered themselves to Henry III. But after nibbling at the offer for eight months Henry was obliged to refuse it. His openly expressed intention to recognise the King of Navarre as his heir had caused a revival of the Holy League. During the winter 1584-5 its reorganisation was busily going on. Philip promised to subsidise it. Mendoza, now ambassador at Paris, was its life and soul. The insurrection was on the point of breaking out. Henry III. knew that the vast majority of Frenchmen were Catholics. To accept the Dutch offer would, he feared, drive them all into the ranks of the Holy League. He therefore dismissed the Dutch envoys with the recommendation that they should apply to England for protection (February 28/March 10, 1585). The manifesto of the Leaguers appeared at the end of March (1585). Henry of Navarre was declared incapable, as a Protestant, of succeeding to the crown. Henry III. was summoned to extirpate heresy. To enforce these demands the Leaguers flew to arms all over France. Had Henry III. been a man of spirit he would have placed himself at the head of the loyal Catholics and fought it out. But by the compact of Nemours he conceded all the demands of the League (June 28/July 7, 1585). Thus began the last great war of religion, which lasted till Henry of Navarre was firmly seated on the throne of France.