Tubularia is not so well suited as hydra to show the influence of
grafting on the united parts, since pieces of tubularia produce
hydranths, both at the oral and aboral ends, although the latter
hydranths take longer to develop. Peebles has shown, nevertheless, that
grafting has an influence on the behavior of a piece. In order to show
that the polarity of a small piece could be affected by a larger piece,
the following experiment was carried out. After cutting off the old
hydranth from the end of a stem, a short piece was then cut from the
distal end of the same stem, turned around, and its oral end brought in
contact with the oral end of the original piece, as indicated in Fig.
49, _F_. The two pieces, being held together for a few minutes, stuck
together and subsequently united perfectly. From eighty-eight pieces
united in this way the following results were obtained. Thirty-six
formed a single hydranth at the end at which the grafting had been made.
The distal row of tentacles appeared in the smaller reversed component,
the proximal row in the larger piece (Fig. 49, _B_). The new hydranth
pushed out later through the perisarc of the smaller piece (Fig. 49,
_C_). In this experiment the smaller component was shorter than the
average length of the hydranth-forming region. In two cases, in which
the smaller component was larger, both circles of tentacles appeared in
this piece. In six of the experiments the tips of the proximal tentacles
arose from a part of the wall of the smaller piece, hence these
tentacles had a double origin (Fig. 49, _F_). In five of the unions the
smaller as well as the larger component produced a hydranth; the two
were stuck together by their oral ends (Fig. 49, _D_, _E_). The
remaining four unions gave somewhat different results. In three of these
the smaller piece produced only a part of a hydranth that remained
sticking to the end of the hydranth formed by the larger component. In
the thirty-six cases in which the minor component took part in the
formation of the single hydranth, the influence of the larger component
was shown not only in reversing the polarity of the smaller component,
although this might in part be accounted for by the closing of the oral
end of the smaller piece, but also in the time of development, since the
hydranth appeared sooner than does the aboral hydranth and at the same
time as does the oral hydranth.
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