no doubt other modifying influences must be also taken into account; but
it is important that attention should be drawn to this side of the
subject.
Grafting experiments in planarians have so far been carried out in only
the two cases which I have described. In one of these the anterior ends
of two short pieces of _Bipalium kewense_ were united (Fig. 50, _A_).
Neither piece produced a head at the region of union. Later the pieces
were cut apart by an oblique cut that passed across the line of union
(Fig. 50, _C_), so that each piece retained at its most anterior end (at
one side) a piece of the other individual in a reversed position. A head
developed at the anterior (and lateral) end of each piece, in such a way
that a part at least of the small reversed piece was contained in the
new head (Fig. 50, _D_). In the other case two pieces of bipalium were
united by their posterior cut-surfaces. Each piece produced a new head
at its free end, and the pieces greatly elongated, but remained sticking
together (Fig. 51).
[Illustration: FIG. 50.--_A._ Two pieces of _Bipalium kewense_ united by
anterior ends. _B, C._ Later stages of same. Line in _C_ indicates how
pieces were cut apart. _D._ Two worms produced by these pieces. All
drawn to scale.]
[Illustration: FIG. 51.--Two pieces of _Bipalium kewense_ united by
posterior ends. Each regenerated a head at anterior end.]
A large number of experiments have been made by Joest in grafting pieces
of earthworms. The cut-surfaces were held in contact by means of two or
three threads passing through the body wall of each piece and tied
across, so that the pieces were drawn together and held firmly in that
position. Joest found that pieces of the same or of different
individuals could be united in various ways, and the union become
permanent. If the anterior end of one worm is united to the posterior
end of the same, or of another worm, a perfect union is formed, and no
subsequent regeneration takes place (Fig. 52, _A_). Long worms can be
made by uniting two pieces, each more than half the length of a worm, or
by uniting three pieces, as shown in Fig. 52, _C_. Short worms can be
formed by cutting a middle piece from a worm, and uniting the anterior
and posterior pieces (Fig. 53, _D_). Joest found that when a short worm
is made in this way, so that no reproductive region is present, the new
worm does not produce new reproductive organs. It is conceivable that
new reproductive organs might have been produced either in the old
segments, or by the formation of a new reproductive region between the
two united pieces, but neither process takes place. In the long worms
two sets of reproductive organs, etc., are present. This sort of union
is, however, less permanent, as the worms often pull apart.
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