Born found that if the anterior half of one tadpole was united to the
posterior half of the same or of another tadpole a single individual was
formed which he kept alive in several cases until the time of
metamorphosis. If the head of a tadpole is cut off and grafted upon the
side of the body of another tadpole, the head will remain alive and
continue to develop in its new position, and, if well nourished by means
of the connecting blood vessels that develop, it may grow to be as large
as the head of the tadpole to which it is attached. Similarly, if the
tail of one tadpole is grafted upon the side of the body of another
tadpole, it also continues to develop, and at the time of metamorphosis,
when the normal tail is absorbed, the additional or misplaced tail also
shows signs of breaking down. Even the posterior half of one tadpole, if
grafted to the ventral side of another, may continue to develop,
producing legs, etc.
Born succeeded in uniting tadpoles of different species in several
different ways. They were united by their heads or by their ventral
surfaces, or longer and shorter tadpoles made by using pieces longer or
shorter than a half. In all of these cases there is no regeneration at
the place of union, and the internal organ, the digestive tract, nervous
system, and blood vessels unite when brought into contact. When pieces
are united end to end, like organs unite to like, the nerve-cord with
the nerve-cord, digestive tract with digestive tract, segmental duct
with segmental duct, cœlom with cœlom, and although less often, the
notochords sometimes join together. The lack of union of the ends of the
notochord is explained by its frequent partial displacement at the
cut-end, for when the cut is made the notochord, being tougher than the
other structures, is often dragged out of place in one or in both
pieces, so that the ends do not meet when the pieces are put together.
When like organs are brought together the substance of one unites
directly with the substance of the other, and if the organ is a hollow
one, as is the digestive tract or the nerve-cord, their cavities also
become continuous. There is also, Born states, some evidence to show
that if similar organs are not brought exactly in contact their ends
find each other and unite, and if they do not at first meet squarely
they may do so later. When the ends of unlike organs are brought in
contact, as, for instance, the nerve-cord and notochord, they do not
unite, but connective tissue develops between them. The union of like
parts, Born suggests, may be due to some sort of cytotropism, the
outcome of a mutual attraction between similar cells like that which
Roux has observed between the isolated cells of the segmented egg of the
frog. Born thinks that the first rapid union of the pieces is due to the
attraction of the ectoderm of one component for that of the other.
Born succeeded also in uniting pieces of the tadpoles of different
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